BMCMC法分析2009年墨西哥H1N1流感病毒与中国流感病毒的进化关系

从GenBank上下载所有1978—2009年在中国分离的H1N1亚型流感病毒的HA和NA基因及所有同期在中国分离的A型流感病毒(宿主包括人、猪和禽)的PB1、PB2、PA、NS、NP和M基因的序列,将这些基因序列与2009年在中国发生的墨西哥H1N1流感病毒的对应基因序列进行BMCMC法分析,绘制BMCMC进化树,结果揭示中国发生的墨西哥H1N1流感病毒的HA和NA基因相对独立于中国季节性H1N1流感病毒;M基因相对独立于中国国内的季节性流感病毒和禽源流感病毒;PB1基因包含在季节性流感病毒的分群内;PB2基因和NS基因与国内重排的流感病毒相关,但相对独立于国内的季节性流感病毒和禽源流感病毒;PA基因包含在国内禽流感病毒的分群内;NP基因则与国内传统的猪流感病毒相关,但相对独立于国内的季节性流感病毒和禽源流感病毒.

Phylogenetic Analysis of the Relationship Between A Influenza Virus H1N1(2009) and Other A Influenza Virus in China by BMCMC

The HA and NA gene sequences of Chinese H1N1 subtype viruses and the sequences of all the rest genes of the Chinese A influenza viruses from different hosts (including human,pig and poultry) collected during 1978-2009 were downloaded from GenBank.All these sequences were aligned against the sequences of 2009 H1N1 viruses in China,and the phylogenetic trees were reconstructed by BMCMC.The result showed that HA and NA genes of 2009 H1N1 viruses in China were relatively different from Chinese seasonal H1N1 subtype viruses.M gene of H1N1 viruses were relatively different from Chinese seasonal flu viruses and avian flu,PB1 gene of 2009 H1N1 viruses were included in the cluster of Chinese seasonal flu viruses,PB2 gene and NS gene of 2009 H1N1 viruses were probably associated with Chinese reassortant flu viruses but relatively different from Chinese seasonal flu viruses and avian flu viruses,PA gene of 2009 H1N1 viruses were included in the cluster of Chinese avian flu viruses,NP gene of 2009 H1N1 viruses were probably associated with Chinese classical swine flu viruses but relatively different from Chinese seasonal flu viruses and avian flu viruses.