| 新生牛血清中大肠杆菌噬菌体检测方法的建立 |
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| 引用本文： | 庄金秋,王艳,梅建国,沈志强.新生牛血清中大肠杆菌噬菌体检测方法的建立[J].中国奶牛,2012(4):38-40. |
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| 作者姓名： | 庄金秋 王艳 梅建国 沈志强 |
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| 作者单位： | 山东省滨州畜牧兽医研究院,滨州,256600 |
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| 摘 要： | 本研究用大肠杆菌标准噬菌体株选择确定的大肠杆菌C-3000作为宿主菌,采用增殖法和噬斑法与双层琼脂平板法,对多个厂家的新生牛血清进行了大肠杆菌噬菌体检测,建立了兽用生物制品细胞培养用新生牛血清中大肠杆菌噬菌体的检测方法。
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| 关 键 词： | 细胞培养 新生牛血清 大肠杆菌噬菌体 检测 |
Establishment and Application of the Detection Method of E.coli Phage from Newborn Calf Serum Used for Cell Culture |
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| ZHUANG Jin-qiu,WANG Yan,MEI Jian-guo,SHEN Zhi-qiang.Establishment and Application of the Detection Method of E.coli Phage from Newborn Calf Serum Used for Cell Culture[J].China Dairy Cattle,2012(4):38-40. |
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| Authors: | ZHUANG Jin-qiu WANG Yan MEI Jian-guo SHEN Zhi-qiang |
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| Institution: | (Shandong Binzhou Animal Science ＆ Veterinary Medicine Academy, Binzhou 256600) |
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| Abstract: | Bacteriophage plaque and proliferation method and double-layer agar plate method were established using E.coli 3000 identified by the standard strains of E.coli phage as hosts. Many kinds of newborn calf serum from different manufacturers were detected. The newborn calf serum as the nutrients of cells were used to make veterinary biological products. |
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| Keywords: | Cell culture Newborn calf serum E coli phage Detection |
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