猪卵丘扩展与卵母细胞核成熟关系的研究

 以猪卵丘卵母细胞复合体（COC）为实验对象研究了猪卵丘扩展与卵母细胞核成熟之间的关系,结果表明,（1）全程培养均暴露于激素环境中不利于卵丘的扩展;（2）卵丘扩展良好与卵丘扩展不好的卵母细胞核成熟比例无统计学差异（P>005）;（3）去除卵母细胞的COC培养后,卵丘细胞仍然能发生扩展;（4）培养24h后去掉COC的卵丘细胞不仅不影响卵母细胞的核成熟,而且极体完整的卵母细胞数目还显著增多（P<0.01）;但去颗粒细胞组卵母细胞的质膜不如对照组光滑;（5）带有壁颗粒细胞（mural granulosa cell,MGC）的COC扩展情况明显优于不带MGC的,其中含有极体的成熟卵数也显著高于后者（P<0.01）。

The Relationship Between the Oocyte Nuclear Maturation and the Cumulus Expansion

The relationship between the oocyte nuclear maturation and the cumulus expansion was studied. The results are as follows: (1) The cumulus expansion was affected negatively when oocyte-cumulus complexs were exposed to maturation medium containing hormone during the culture period; (2) There was no obvious relationship between oocyte nuclear maturation and the cumulus expansion grade; (3) The cumulus still underwent expansion even though the oocyte was removed microsurgically; (4 ) The removal of cumulus cells after 24 hours of culture had no effect on oocyte nuclear maturation; but the proportion of oocyte with intact-Pb in the treated group was obviously higher than in the control group (P<0. 01) and the quality of oocye membrane in experimental group was also better than in the control; (5) The COC with some mural granulosa cells showed a better expansion grade and a higher rate of oocyes with intact-Pb than those COC cultured without mural granulosa cells.