苹果组培苗离体叶片诱导不定芽分化

以福早红、鲁加3号和鲁加6号3个苹果品种的组培苗叶片为试材,研究了植物生长调节剂、基因型、暗培养、AgNO3对苹果叶片不定芽分化的影响.结果表明:福早红、鲁加3号和鲁加6号3个苹果品种的组培苗叶片分化最佳培养基分别为MS+6-BA 4 mg/L+NAA 0.20 mg/L,MS+6-BA 4 mg/L+NAA 0.10 mg/L,MS+6-BA 4 mg/L+NAA 0.05 mg/L;基因型是决定苹果组培苗叶片分化的重要因素,3个苹果品种叶片分化不定芽的能力从大到小依次是福早红、鲁加6号、鲁加3号;暗培养可以明显促进苹果叶片进行脱分化,提高叶片的分化率,苹果叶片暗培养处理的最佳时间为15 d;AgNO3抑制苹果叶片愈伤组织的形成,不能促进其叶片不定芽的形成.

Adventitious shoots regeneration of leaves in vitro from apple grown in tubes

Three apple cultivars named Fuzaohong, Lujia 3 and Lujia 6 were used to study the effects of different plant growth regulators, genotypes, dark treatment and AgNO_3 on adventitious shoot regeneration of leaves from apple grown in tubes. The results showed that the optimum medium of adventitious shoot regeneration for Fuzaohong, Lujia 3 and Lujia 6 apples respectively were MS+6-BA 4 mg/L + NAA 0.20 mg/L, MS+6-BA 4 mg/L + NAA 0.10 mg/L, MS+6-BA 4 mg/L + NAA 0.05 mg/L. The genotype was key factor on adventitious shoot regeneration of apple leaves. The ability of leaf regeneration frequency of Fuzaohong apple was highest, but that of Lujia 3 was lowest. Dark treatment could increase obviously the ability of adventitious shoot regeneration and promote redifferentiation of apple leaves. The optimal time of dark treatment was 15 days. AgNO_3 could restrain formation of callus, but couldn't promote the adventitious shoot regeneration of leaves.