Abstract: | Inter Simple Sequence Repeat (ISSR) was used to detect genetic variation among nine provenances, including 135 individuals.
A total of 108 loci were amplified using 10 random primers. The differentiation of the percentage of polymorphic bands (PPB)
among different provenances was evident, ranging from 27% to 54%, of which Honghuaerji provenance had the highest PPB and
Kalunshan provenance had the lowest PPB. Shannon's Information index (I) at species level was 0.1581 and Nei's gene diversity
(h) was 0.2393. Coefficient of gene differentiation (GST) calculated by Popgene was 0.3965, these results indicated that majority of genetic variation (60.35%) was found within provenances.
According to dendrogram amongpinus sylvestriv provenances, nine provenances were divided into two provenance areas, namely Daxing's and Xiaoxing'an Mountains provenance
area and Hulunbeier provenance area.
Foundation item: This study was supported by National Scientific and Technological Brainstorm Project.
Biography: LI Hui-yu (1978-), female, Ph.D. candidate, Northeast Forestry University, Harbin 150040, P. R. China.
Responsible editor: Song Funan |