| 儿茶素EGCG对Caco-2细胞α-葡萄糖苷酶活性的抑制作用研究 |
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| 引用本文： | 刘淑媛,艾仄宜,陈玉琼,倪德江.儿茶素EGCG对Caco-2细胞α-葡萄糖苷酶活性的抑制作用研究[J].湖北农业科学,2017,56(13). |
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| 作者姓名： | 刘淑媛 艾仄宜 陈玉琼 倪德江 |
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| 作者单位： | 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室,武汉,430070 |
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| 摘 要： | 试验对Caco-2细胞膜上蔗糖酶和麦芽糖酶这两种α-葡萄糖苷酶活性进行不同培养时间检测,并以儿茶素单体EGCG作为抑制剂,探究其对Caco-2来源的蔗糖酶和麦芽糖酶的抑制效果。结果表明,Caco-2细胞培养18 d后膜表面具有较高活性的蔗糖酶和麦芽糖酶,可用于进行α-葡萄糖苷酶抑制剂筛选。儿茶素EGCG具有蔗糖酶和麦芽糖酶抑制活性,抑制IC50分别为31.08μg/m L和36.44μg/m L。
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| 关 键 词： | 茶 EGCG 蔗糖酶 麦芽糖酶 Caco-2细胞 |
Alpha-glucosidase Inhibitory Activity of EGCG in Caco-2 Cells |
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| LIU Shu-yuan,AI Ze-yi,CHEN Yu-qiong,NI De-jiang.Alpha-glucosidase Inhibitory Activity of EGCG in Caco-2 Cells[J].Hubei Agricultural Sciences,2017,56(13). |
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| Authors: | LIU Shu-yuan AI Ze-yi CHEN Yu-qiong NI De-jiang |
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| Abstract: | This research used the Caco-2 cell monolayer model in vitro to simulate the α-glucosidase activity at different culture days. EGCG was used as an inhibitor to investigate the inhibition activities on sucrase and maltase in Caco-2 cells. The results illustrated that Caco-2 could be used to estimate α-glucosidase inhibitory activity after 18 days with high sucrase and maltase activities. EGCG showed inhibitory effect on sucrase and maltase in Caco-2 cells, with the IC50 values of 31.08μg/mL and 36.44μg/mL, respectively. |
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| Keywords: | tea EGCG sucrase maltase Caco-2 |
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