转基因三倍体毛白杨抗虫性鉴定

【研究目的】转抗虫基因育种是控制害虫发生的有效途径,研究旨在对29个转基因三倍体毛白杨进行抗虫性筛选和抗性鉴定。【方法】通过离体的转基因三倍体毛白杨株系叶片室内饲虫实验,统计死亡指数及死亡率,用DPS统计软件对两年的饲虫数据进行分析。【结论与结果】把这29个株系的抗虫性分为了三类:饲虫死亡率高于85%的1号、17号、41号、21号、7号、8号、27号、6号、11号定为高抗株系,饲虫死亡率在60%～79%的5号、33号、32号、29号、22号和38号定为中抗株系,饲虫死亡率低于60%的26号、39号、31号、30号、16号、36号定为低抗株系。为转基因三倍体毛白杨的进一步研究与推广奠定基础。

Identification of the Insect-resistance of Transgenic Triploid Chinese White Popular

Breeding techniques of transgenic Insect-resistant is the effective way on pest control. The research were trended in selecting and evaluation of tile 29 clones of transgenie triploid Chinese white popular to insect pest. The larvae were fed with the leaves of transgenic triploid Chinese white popular clones in laboratory, and statistics tile death index and mortality of the larvae. DPS statistic software is used to analyzed of the two years data of death index and mortality of the larvae, and the 29 clones of transgenic triploid Chinese white popular were divided into three categories. The Clones 1,17,41,21,7,8,27,6 and 11 were belong to the higher insect-resistance, lhe larvae mortality were all higher than 85%. The Clones 5,33,32,29,22 and 38 were belong to the middle insect-resistance, and the larvae mortality were be- tween 60% and 79%. The Clones 26,39,31,30,16 and 36 were belong to lower inscct-resistance, the larvae mortality were all lower than 60%. The research provide foundation for studying and popularizing of transgenic triploid Chinese white popular.