普洱茶缓解小鼠被动吸烟烟毒危害的研究初报

设计2个试验研究普洱茶缓解被动吸烟小鼠烟毒危害的作用。试验一观察小鼠被动吸烟10～80min的行为,发现60min为建立被动吸烟模型的适合时间。试验二180只小鼠分为3组,空白组不吸烟、灌服生理盐水;试验Ⅰ组吸烟、灌服生理盐水;试验Ⅱ组吸烟、灌服普洱茶。试验周期6周。试验全期,试验组I和II小鼠血清尼古丁浓度显著高于(P<0.01)空白组,d14～42试验II组尼古丁浓度低于(P<0.05)试验I组。试验全期,试验组I和II小鼠血清可的宁浓度差异不显著。本文结果表明,普洱茶显著降低烟毒尼古丁浓度,且时间越长效果越显著,证明普洱茶对缓解烟毒危害有直接效果。

Effects of Oral Administration of Pu'er Tea Extracts on Serum Nicotine and Cotinine Concentrations in Passive Smoking Mice

Two experiments were designed to determine the effects of oral administration of Pu''er tea extracts on serum nicotine and cotinine concentrations in passive smoking mice. In the experiment 1, fifty ICR mice were assigned randomly to one of five treatments to receive cigarette smoking time 10, 20, 40, 60, or 80 min, observed the mice behaviors. The results showed that 60 min was prior in this experiment. In the experiment 2, one hundred and eighty ICR mice were assigned randomly to one of three treatments with 10 mice per group. The control group orally administered physiological saline, group I orally administered physiological saline and received cigarette smoking, and group II orally administered Pu''er tea extracts and received cigarette smoking. The mice serum was sampled on d 7, 14, 21, 28, 35, or 42, and the serum nicotine and cotinine concentrations were determined. The nicotine concentrations of group I and II were higher (P < 0.01) than those of the control during the period of the second experiment, and the nicotine concentrations of group II were lower than those of group I on d 14 (P = 0.01), 21 (P = 0.02), 28 (P < 0.01), 35 (P < 0.01), or 42 (P < 0.01). The cotinine concentrations were no significant difference between group I and II. The findings of this paper showed that oral administration of Pu''er tea extracts could reduce serum nicotine in passive smoking mice.