猪血凝性脑脊髓炎病毒RT-PCR方法的建立及初步应用

为了建立快速检测猪血凝性脑脊髓炎病毒(HEV)的RT-PCR方法,根据GenBank中HEV的HE基因和S基因设计了1对引物,在优化RT-PCR反应条件的基础上,成功的扩增出323bp的特异性条带。检测与HEV亲缘性较高的牛冠状病毒及猪的伪狂犬病毒均为阴性,最低可以检测到10个TCID50/100μl的病毒,说明该方法的有较好的特异性和敏感性。用此RT-PCR方法对感染HEV的小鼠和猪进行检测,结果能从发病动物的多种组织中检测到病原,其中以脑组织的检出率最高。因此,临床疑似病例检测时以脑组织为最佳检测样本。

Establishment of Detection of Hemagglutinating Encephalomyelitis Virus by RT- PCR

OBJECTIVE]The objective of this investigation was to study the effects of NH4 -N/NO3--N ratios on the yield and soluble oxalate content of spinach fresh shoot.METHOD]Spinach seedling was treated with different ratios of NH4 -N/NO3--N,the yield and soluble oxalate content of spinach fresh shoot were observed after 36 days.RESULTS]Results showed that(1)from NH4 -N/NO3--N ratio of 100:0 to the ratio of 0:100,spinach shoot fresh weight gradually increased with the increase of the percent of NO3--N in the total nitrogen.Spinach supplied with NO3--N as its sole nitrogen source had the highest yield;(2)with the decrease of the NH4 -N/NO3--N ratio,the soluble oxalate content of spinach shoot gradually decreased.As the NH4 -N/NO3--N ratio of 25:75,the soluble oxalate content was the lowest,with the ratio further smaller,the oxalate content was reversed to increase.There was a significant quadric relationship between the percent of in the total nitrogen and soluble oxalate content of spinach shoot.CONCLUSION]The yield of spinach was increasing with the NH4 -N/NO3--N ratio increasing,the oxalate content was the highest when the ratio of NH4 -N/NO3--N was 25:75.