31种药用植物HMGR蛋白的生物信息学分析

利用生物信息学方法对大戟(Euphorbia pekinensis)、曼地亚红豆杉(Taxus media)、球药隔重楼(Paris fargesii)等31种药用植物HMGR蛋白质的理化性质、亲/疏水性、跨膜结构、蛋白质二级结构、信号肽等进行预测和分析。结果表明,31种药用植物HMGR蛋白氨基酸残基数量大部分在500~600 aa之间;大多数定位于质膜上;分子质量在58~65 ku之间;主要富含Gly、Ala、Leu、Ser和Val等氨基酸。HMGR蛋白具有一定的亲水性,含有两个跨膜结构域,均不具有信号肽,二级结构的主要构件为α-螺旋和不规则卷曲。曼地亚红豆杉、罗汉果、假马齿苋、苍术、秦艽、土沉香、丹参、银杏、黄花蒿HMGR蛋白为稳定蛋白,而其余均为较不稳定蛋白。同源性分析表明,除了灵芝以外,其他植物均聚集在同一枝上,药用植物HMGR蛋白有较高的保守性。

Bioinformatic Analysis of HMGR Protein of 31 Kinds Medicinal Plants

The physical and chemical properties,Pro/hydrophobicity,transmembrane structure,protein two structure,signal pep-tide and other aspects of the prediction of the HMGR protein of 31 kinds of medicinal plants were analyzed by using bioin-formatics methods and tools,including Euphorbia pekinensis,Taxus media, Paris fargesii and other 31 kinds of medicinal plants. Results showed thatthe number of amino acid residues of HMGR protein of 31 medicinal plants was mostly between 500 aa and 600 aa;Most localized on the plasma membrane;Molecular mass was 58~65 ku;Mainly riched in Gly,Ala,Leu,Ser and Val and other amino acids. HMGR protein has a certain degree of hydrophilicity,which contains two transmembrane do-mains,and has no signal peptide. The main component of the two stage structure was alpha helix and random coil. The HM-GR proteinof Taxus media,Siraitia grosvenorii,Bacopa monnieri,Atractylodes lancea,Gentiana macrophylla,Aquilaria sinensis, Salvia miltiorrhiza,Ginkgo biloba and Artemisia annua were a stable protein,while the rest were less stable protein. In addition to ganoderma lucidum,other plants were gathered on the same branch,which showed that the medicinal plant HMGR protein has a high conservation.