| Aspergillus niger 乳糖酶基因的克隆及序列分析 |
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| 引用本文： | 李淑娟,郭润芳,于宏伟,贾英民. Aspergillus niger 乳糖酶基因的克隆及序列分析[J]. 勤云标准版测试, 2007, 0(6) |
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| 作者姓名： | 李淑娟 郭润芳 于宏伟 贾英民 |
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| 作者单位： | 河北农业大学食品科技学院 保定071001 |
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| 基金项目： | 河北省“十一五”科技攻关项目资助(农产品精深加工用主要微生物菌种培育及菌种资源库建设(06220106D) |
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| 摘 要： | 利用PCR及RT-PCR技术从黑曲霉(Aspergillus niger)菌株DL116中克隆到了乳糖酶基因组DNA,cDNA序列(GENBANK ACCESSION No.EF103141)。序列分析表明,乳糖酶基因组DNA序列长3368bp,其中含有8个内含子,cDNA编码区长2967bp,共编码988个氨基酸,氨基酸序列中共含有12个潜在的糖基化位点。并将此基因与不同来源的乳糖酶基因进行了同源性比较。
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| 关 键 词： | 黑曲霉 乳糖酶基因 克隆 序列分析 |
Cloning and Sequencing of Lactase Gene from Aspergillus Niger |
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| Li Shujuan,Guo Runfang,Yu Hongwei,Jia Yingmin. Cloning and Sequencing of Lactase Gene from Aspergillus Niger[J]. , 2007, 0(6) |
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| Authors: | Li Shujuan Guo Runfang Yu Hongwei Jia Yingmin |
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| Affiliation: | College of Food Science and Technology, Agricultrural University of Hebei, Baoding 071001 |
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| Abstract: | Genomic DNA and cDNA sequences of lactase from Aspergillus niger DL116 were cloned by PCR and RT-PCR. Sequences analysis revealed that the genomic DNA was 3368bp containing eight introns, cDNA was 2967bp,encoding a protein of 988 amino acids,12 potential N-glycosylation sites were assumed. (GENBANK ACCESSION No.EF103141). The gene and amino acid sequences were compared with other lactase sequences from various sources. |
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| Keywords: | Aspergillus niger Lactase gene Clone Sequence analysis |
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