自然孵化与换壳培养鸭胚胎碱性磷酸酶活性的研究

地黄花叶病毒(Rehmannia mosaic virus,ReMV)是地黄上发生的一种烟草花叶病毒属(Tobamovirus)可能的新病毒。笔者对ReMV的生物学特性进行了初步研究。结果表明,ReMV粒子形态为杆状,可以侵染茄科、番杏科、十字花科和藜科等4科10种植物,其中在茄科的心叶烟、黄花烟、蔓陀罗;番杏科的番杏;藜科的苋色藜和昆诺藜上表现为局部枯斑症状。在红花烟、普通烟、番茄和白菜等植物上表现为系统花叶症状。ReMV的钝化温度为90-95℃,稀释限点为10-5-10-6,20-22℃条件下的体外存活期在60d以上。

Study on the Activity of Alkaline Phosphatase in Duck Embryos with Normal Incubation and Culture in Surrogate Eggshells

The effect of alkaline phosphatase (ALP) on calcium metabolism of duck embryo was studied in present experiment The activity of ALP in the bone and plasma and the specific activity in the bone of embryos was tested at the incubated day of 14th, 16th, 18th, 20th, 22nd, 24th, 26th, 28th in the surrogate eggshell cultured group (treatment) and normal one (control). The result showed that the activity of ALP increased significantly from the day of 14th to the day of 28th (P<0.01) both in treatment and in control, but the activity was significantly higher in the former than the latter with the same cultured time (P<0.01). The specific activity in the bone of embryos was higher in treatment than in control after 22nd, like the variation of ALP activity. The result indicated that activity of ALP in the bone and plasma could elucidate the calcium metabolism of the embryos and showed that calcium deficiency was appeared in the later development period of embryo in the surrogate eggshell group.