| 土壤甲烷单加氧酶活性测定的研究 |
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| 引用本文： | 王静,夏芳芳,王闻伟,何若,沈东升.土壤甲烷单加氧酶活性测定的研究[J].土壤通报,2011(3):589-592. |
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| 作者姓名： | 王静 夏芳芳 王闻伟 何若 沈东升 |
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| 作者单位： | 浙江大学环境与资源学院;台州市环境科学设计研究院;浙江工商大学环境科学与工程学院; |
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| 基金项目： | 浙江省自然科学基金项目(Y5080154); 浙江省教育厅项目(20070055)资助 |
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| 摘 要： | 以垃圾生物覆盖土、垃圾填埋场覆盖土、菜地土为材料,研究了土壤甲烷单加氧酶(Methane monooxygenase,MMO)活性的测定条件。结果表明:0.04 g kg-1 NaN3含量可抑制土壤生物活性,可作为土壤MMO活性测定时的对照;最适的底物丙烯浓度可为0.5%;培养时间对土壤MMO活性测定影响不大,但从测定的误差和可靠性考虑,可选用5 d作为土壤MMO活性测定的培养时间。统计分析表明:采用该方法测定的土壤MMO活性与其甲烷氧化潜力有很好的线性关系。
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| 关 键 词： | 甲烷单加氧酶 甲烷氧化 土壤甲烷单加氧酶活性 |
Study on the Determination of Soil Mathane Monooxygenase Activity |
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| WANG Jing ,XIA Fang-fang ,WANG Wen-wei ,HE Ruo ,SHEN Dong-shen.Study on the Determination of Soil Mathane Monooxygenase Activity[J].Chinese Journal of Soil Science,2011(3):589-592. |
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| Authors: | WANG Jing XIA Fang-fang WANG Wen-wei HE Ruo SHEN Dong-shen |
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| Institution: | WANG Jing 1,XIA Fang-fang 1,WANG Wen-wei 2,HE Ruo 1,SHEN Dong-shen 3(1.College of Environment and Resources,Zhejiang University,Hangzhou 310029,China,2.Taizhou City Design and Research Institute of Environmental Science,Taizhou 318000,3.College of Environmental Science and Engineering,Zhejiang Technology and Business University,Hangzhou 310012,China.) |
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| Abstract: | The conditions for measuring soil methane monooxygenase(MMO) activity were studied using waste biocover soil,landfill cover soil and vegetable soil.The results showed that 0.04 g kg-1 NaN3 inhibited soil biological activity,which could be used as control for the analysis of MMO activity.0.5% propylene was a suitable substrate concentration.The incubation time didn`t have an obvious effect on the measurement of soil MMO activity,but as far as measurement deviation and reliability were concerned,5 days' incub... |
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| Keywords: | Methane monooxygenase(MMO) Methane oxidation Soil MMO activity |
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