| 响应面法优化飞机草总黄酮提取工艺 |
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| 引用本文： | 王桂荣,张燕娣,张连学. 响应面法优化飞机草总黄酮提取工艺[J]. 北方园艺, 2012, 0(15): 78-82 |
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| 作者姓名： | 王桂荣 张燕娣 张连学 |
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| 作者单位： | 吉林农业大学中药材学院,吉林长春,130118;吉林农业大学中药材学院,吉林长春,130118;吉林农业大学中药材学院,吉林长春,130118 |
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| 摘 要： | 采用溶剂回流法从飞机草中提取总黄酮。单因素试验以提取温度、提取时间、料液比和乙醇浓度作为考察因素,Box-Behnken中心组合试验法设计,采用响应面法(RSM)评估了4个因素对飞机草总黄酮得率的影响。结果表明:响应面的典型分析可知稳定点是最大值,决定系数为0.9722。飞机草黄酮最佳提取工艺条件为提取温度80℃、提取时间4.5h、料液比1∶41、乙醇浓度51%。在此条件下飞机草黄酮的得率为7.162%。
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| 关 键 词： | 飞机草 黄酮类化合物 响应面法 |
Optimization of Extraction Technology for Total Flavonoids from Eupatorium odoratum L.by Response Surface Methodology |
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| WANG Gui-rong , ZHANG Yan-di , ZHANG Lian-xue. Optimization of Extraction Technology for Total Flavonoids from Eupatorium odoratum L.by Response Surface Methodology[J]. Northern Horticulture, 2012, 0(15): 78-82 |
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| Authors: | WANG Gui-rong ZHANG Yan-di ZHANG Lian-xue |
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| Affiliation: | (College of Chinese Medicinal Materials,Jilin Agriculture University,Changchun,Jilin 130118) |
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| Abstract: | Response surface methodology(RSM) was applied to optimize extraction process of total flavonoids from Eupatorium odoratum L.Based on single experiments,extraction temperature,extraction time,ratio of liquid to solid and ethanol concentration were selected for Box-Behnken central composite designing.RSM was employed to study the effect of these factors on the yield of total flavonoids.The results showed that canonical analysis of surface responses revealed the stationary surface was a maximum.The coefficient of determination was 0.9722.The optimal process conditions were extraction temperature 80℃,extraction time 4.5 hours,ratio of liquid to solid 1∶41 and ethanol concentration 51%.Under these optimized conditions,the extraction yield of total flavonoids was up to 7.162%. |
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| Keywords: | Eupatorium odoratum L. flavonoids response surface methodology |
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