超声波辅助盐析提取猪小肠中肝素的工艺

为研究超声波处理对肝素提取效果的影响，并找出最佳的处理技术参数。采用商业屠宰猪的小肠，用超声波辅助盐析法提取肝素，在超声功率、超声时间、超声温度和液料比4个单因素试验的基础上，采用四因素二次正交旋转组合设计来优化工艺参数。试验结果表明，在超声功率300 W，超声温度40℃，超声时间40 min，液料比为15 mL/mg条件下肝素得率最高，达到0.0184%，比盐析法的得率提高了10%以上，节省提取时间2 h左右。试验结果表明，超声波技术在肝素提取工艺中具有较高的应用价值，可作为一种新型工艺技术进行推广。

Process of heparin extraction using ultrasonic assisted salting out

The effects of ultrasonication on heparin extraction were studied, and the technological parameters were optimized. Porcine intestines of commercial slaughtered were treated with ultrasonic assisted salting out to extract heparin. Based on the single factor tests for ultrasonication power, ultrasonication temperature, ultrasonication time, and the ratios of solution to material, a four-variable quadratic orthogonal rotation combination experimental design was applied to optimize the parameters for ultrasonic extraction heparin. The results indicated that the heparin yield was the highest under the conditions which ultrasonication power was set at 300 W, ultrasonication temperature 40℃, ultrasonication time 40 min, and ratio of solution to materials was 15 mL/mg. According to these conditions, the heparin yield was 0.0184%. Which was 10% more than the yield of salting out extraction, and the extraction time was decreased by 2 h. The results suggested that the ultrasonic wave extraction process was feasible, and the extraction yield of heparin was higher than the conventional salting out method, therefore, research on this technology was significant important for practical application.