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燕麦DNA导入普通小麦的初步研究
引用本文:刘萍 李树华. 燕麦DNA导入普通小麦的初步研究[J]. 麦类作物学报, 2000, 20(4): 6-11
作者姓名:刘萍 李树华
作者单位:1. 宁夏农学院农学系,永宁,750105
2. 宁夏农林科学院,银川,750002
基金项目:宁夏回族自治区科委 1998~ 2 0 0 0年项目
摘    要:以健壮燕麦(Avena sativa L.)为供体,宁春4号小麦为受体,采用花粉管通道法进行外源DNA导入。结果表明,变异株系在生育期、株高、穗长、结实小穗数、千粒重等性状上产生了明显的变异;酯酶和过氧化物酶同工酶谱带数与受体相比增加或减少,叶绿素含量和瞬间光合速率偏向供体或受体;并筛选到对小麦条锈病免疫或高抗的部分变异株,表明燕麦DNA已导入到小麦中,并得到表达。

关 键 词:燕麦 外源DNA导入 宁麦4号 性状变异 小麦

Preliminary Study on the Introduction of Oats ( Avena sativa L.) Exogenous DNA into Wheat and Its Variation of Characteristics
LIU Ping,ZHANG Li jie,MA Hong wei,LIU Sheng xiang,XU Zhao zhen,LI Shu hu,XU Xing. Preliminary Study on the Introduction of Oats ( Avena sativa L.) Exogenous DNA into Wheat and Its Variation of Characteristics[J]. Journal of Triticeae Crops, 2000, 20(4): 6-11
Authors:LIU Ping  ZHANG Li jie  MA Hong wei  LIU Sheng xiang  XU Zhao zhen  LI Shu hu  XU Xing
Abstract:Total DNA introduction was studied by using pollen tube passage way with oats ( Avena sativa L.) as donor and wheat as receptor. The growing period, plant height, ear length, spikelets per spike and 1000-grain weight varied abviously from first generation (D 1). The esterase isoenzyme and peroxidase isoenzyme pattern of variant strains showed difference. Several new variant strains obtained immune or high resistant to wheat stripe rust. The results indicated that the exogenous DNA of oats had been introduced into the wheat.
Keywords:Oats ( Avena sativa L.)  Exogenous DNA introduction  Pollen tube passage  Spring wheat  Variation in characters
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