山绿茶总黄酮对小鼠高脂血症模型调脂作用的研究

取ICR小鼠,饲喂高脂饲料,以建立高脂血症动物模型,用山绿茶总黄酮[300、150、75 mg·(kg·d)~(-1)]连续灌胃小鼠28 d,检测模型小鼠血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C);取肝脏制作切片,观察组织结构.结果表明:高脂血症模型小鼠的TC、TG、LDL C含量明显高于正常组,HDL-C含量低于正常组;肝细胞中充满大量脂肪颗粒,细胞核呈固缩状.与模型组相比,山绿茶总黄酮高、中剂量均能一定程度降低血清LDL-C含量、提高HDL含量,差异极显著;山绿茶总黄酮能减少肝细胞质中脂肪颗粒及炎性细胞;部分细胞核无固缩现象,较模型组其形态结构有所改善.说明山绿茶总黄酬在一定程度上可降低高脂血症模型小鼠LDL-C含量、提高HDL含量,对血脂水平有调节作用;可改善肝细胞脂肪变性的形态结构.

Effect of hainan holly leaf total flavonoids on serum lipids based on hyperlipidemia mice

ICR mice were fed with high fat diet to establish hyperlipidemia model.Three dosage groups of hainan holly total flavonoids[300,150,75 mg·(kg·d)~(-1)]were intragastric administrated for 28 d to detect triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C);Part of liver tissue was taken to make slices for observing liver's morphological structure.The data showed that levels of TC,TG,LDL-C of the normal group were remarkably higher than the normal group;and HDL-C level was lower than the normal group.Liver cells were full of fat particles and cell nucleus condensed.Compared with the model group,the highmid-dose of hainan holly leaf total flavonoids both could significantly increase HDL-C level(P<0.01);Effect hainan holly leaf could decrease fat particles and Inflammatory cells in liver cytoplasm;part of cell nucleus don't condense.compared with the model group,it could improve cell nucleus's morphological structure.It is suggested that hainan holly leaf total flavonoias can decrease TG,LDL-C levels based on hyperlipidemia mice,to some extent and can improve lipoprotein level and Steatosis liver's morphological structure.