Determination of biogenic amines in aerobically stored beef using high-performance thin-layer chromatography densitometry |
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| Authors: | S Li M Johansson J K Vidanarachchi J Pickova G Zamaratskaia |
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| Institution: | 1. School of Food Science and Technology, Dalian Polytechnic University;2. National Engineering Research Center of Seafood, Dalian, People’s Republic of China;3. Department of Molecular Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden;4. College of Food Science and Technology, Bohai University, Jinzhou, People’s Republic of China;5. Department of Molecular Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden;6. Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka |
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| Abstract: | The aim of this study was to develop a high-performance thin-layer chromatography densitometry method to simultaneously evaluate levels of different biogenic amines (BAs) in beef stored aerobically at 4°C. Steaks from M. longissimus thoracis et lumborum muscles were overwrapped with polyvinyl chloride film and stored for 0, 4, 7, and 10 days. For this purpose, the solvent system and pH for the derivatization of BAs with dansyl chloride were optimized. Moreover, this method was also validated for linearity, limits of detection and quantification, precision, and recovery. As found using this method in beef samples, spermine content decreased significantly after 7 days of storage, and tyramine and cadaverine were not detected until day 10. |
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| Keywords: | Biogenic amine HPTLC beef spoilage |
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