枣幼胚子叶再生植株的研究

本试验研究了影响金丝小枣幼胚子叶再生植株的相关因素,获得了金丝小枣幼胚子叶的再生植株。正交试验结果表明:金丝小枣幼胚子叶在附加TDZ0.6mg/L、IAA0.5mg/L、NAA0.3mg/L的1/2MS培养基中,暗培养20d,愈伤组织诱导率和再生频率均最高,分别达到100%和41.86%。再生获得的不定芽在MS培养基上,添加6-BA1.0mg/L和IBA0.1mg/L,增殖效果最好;在1/2MS+IBA1.0mg/L培养基上,生根率达到100%。

PLANT REGENERATION FROM COTYLEDON OF IMMATURE EMBRYO OF JUJUBE

The effects of TDZ,IAA,NAA and medium on bud initiation of cotyledon of immature embryo from jujube cultivar Jinsixiaozao were evaluated by L_9(34)orthogonal design.The result showed that TDZ was the most important factor for bud initiation followed by medium,IAA,and NAA.The optimum combination medium was on 1/2MS medium containing TDZ(0.6mg/L),IAA(0.5mg/L)and NAA0.3(mg/L)by variance analysis,on which cultured in dark 20d,callus inducing rate was 100% and adventitious bud inducing rate was 41.86%.The optimum media for adventitious bud propagation and for root inducing are on MS medium adding 6-BA 1.0mg/L,IBA 0.1mg/L and on 1/2MS medium supplemented with IBA 1.0mg/L respectively.