~(60)Coγ射线对双低甘蓝型油菜(Brassica napus L.)的辐射效应

用~(60)Coγ射线0、80、100、120、140、160krad的剂量辐射处理3个双低(低芥酸、低硫甙)甘蓝型油菜品种的干种子,从形态学、细胞学和生物化学3个方面研究了M_1代的剂量效应。结果表明,田间出苗率、成株率、产量、自交结果率、角果长度、每果粒数、自交结籽率和粗脂肪含量与剂量拟合曲线负相关,形态畸变率和染色体畸变率与剂量拟合曲线正相关,种子的芥酸和亚麻酸含量、菜饼的硫甙和蛋白质含量随着剂量的增加而呈不规则性变化。形态学效应、细胞学效应和生物化学效应之间关系密切,相关显著。3个品种的辐射敏感性顺序为中双2号>中双1号>中双3号。

THE EFFECTS OF GAMMA IRRADIATION ON MORPHOLOGICAL, CYTOLOGICAL AND BIOCHEMICAL CHARACTERS OF DOUBLE-LOWRAPE (Brassica napus L.)

The dormant seeds of three varieties of double-low rape were treated with 0,80,100,120,140 and 160 krad of γ-ray.The dose-effect curves of survival rate,yield,length of pod,rate of bearing pods and oil content in rapeseed fit the equation:y= Exp (-Ax-Bx2).The dose-effect curves of field germination rate,seed bearing rate,number of seeds per pod and chromosome aberration rate fit the equation:y = A + Bx.The dose-effect curve of morphological malformation rate fit the equation:y = AeBx The content's of erucic acid,linolenic acid,glucosino-lates and protein in rapeseed,changed irregularly with the increase of doses of gamma irradiation.Close correlation were found among the effects of irradiation on morphological,cytological and biochemical characters.The radiosensitivities of rape varieties were apparently different.