A genetic map of macadamia based on randomly amplified DNA fingerprinting (RAF) markers |
| |
| Authors: | C.P. Peace V. Vithanage C.G.N. Turnbull B.J. Carroll |
| |
| Affiliation: | (1) CSIRO Plant Industry, 120 Meiers Rd, Indooroopilly, Qld, 4068, Australia;(2) Department of Botany, University of Qld, St Lucia, Qld, 4067, Australia;(3) School of Molecular and Microbial Sciences and School of Land and Food Sciences, University of Qld St Lucia, Qld, 4067, Australia;(4) Present address: Department of Pomology, UC Davis, Kearney Agricultural Center, 9240 S. Riverbend Ave., Parlier, CA, 93648, U.S.A |
| |
| Abstract: | The first genetic linkage map of macadamia (Macadamia integrifolia and M. tetraphylla) is presented. The map is based on 56 F1 progeny of cultivars ‘Keauhou’ and ‘A16’. Eighty-four percent of the 382 markers analysed segregated as Mendelian loci. The two-way pseudo-testcross mapping strategy allowed construction of separate parental cultivar maps. Ninety bridging loci enabled merging of these maps to produce a detailed genetic map of macadamia, 1100 cm in length and spanning 70–80% of the genome. The combined map comprised 24 linkage groups with 265 framework markers: 259 markers from randomly amplified DNA fingerprinting (RAF), five random amplified polymorphic DNA (RAPD), and one sequence-tagged microsatellite site (STMS). The RAF marker system unexpectedly revealed 16 codominant markers, one of them a putative microsatellite locus and exhibiting four distinct alleles in the cross. This molecular study is the most comprehensive examination to date of genetic loci of macadamia, and is a major step towards developing marker-assisted selection for this crop. This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
| Keywords: | genetic map heterozygosity macadamia RAF two-way pseudo-testcross |
| 本文献已被 SpringerLink 等数据库收录！ |
|
