| 宝巾(Bougainvillea spp.)种质资源同工酶的遗传多样性分析 |
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| 引用本文： | 陈庭,刘伟,谢良生,雷江丽.宝巾(Bougainvillea spp.)种质资源同工酶的遗传多样性分析[J].安徽农业科学,2012,40(1):29-31. |
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| 作者姓名： | 陈庭 刘伟 谢良生 雷江丽 |
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| 作者单位： | 深圳市仙湖植物园管理处,广东深圳,518004;华南农业大学生命科学学院,广东广州,510642 |
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| 基金项目： | 深圳市科技局基础研究项目 |
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| 摘 要： | 目的]分析深圳本地12种宝巾种质资源的遗传多样性。方法]利用聚丙烯酰胺凝胶电泳法研究宝巾种质资源过氧化物同工酶(POD)和酯酶同工酶(EST)的遗传多样性,并对统计结果进行聚类分析。结果]各宝巾种质资源间有丰富的遗传多样性,在相似系数为0.63水平上可以聚为2大类。结论]同工酶的特征谱带应用于品种鉴定是可行的,在一定程度上明确了各宝巾品种的亲缘关系。
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| 关 键 词： | 宝巾 遗传多样性 同工酶 过氧化物酶 酯酶 聚类分析 |
Analysis on Genetic Diversity of Bougainvillea spp.Germplasm Resources Based on Isozyme |
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| Institution: | CHEN Ting et al(Shenzhen Fairy Lake Botanical Garden,Shenzhen,Guangdong 518004) |
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| Abstract: | Objective] The aim was to analyze the genetic adversity of 12 Bougainvillea spp.germplasm resources from Shenzhen region.Method] The esterase(EST) isozyme and peroxidase(POD) isozyme of Bougainvillea spp.germplasm resources were determined by polyacrylamide gel electrophoresis and the cluster analysis was used according to statistical results.Result] There was great genetic adversity among different germplasm resources and the resources could be divided into two groups based on 0.63 of similarity coefficient.Conclusion] The characteristic band of isozyme was suitable for variety identification and defining the blood relationship for various varieties to some extent. |
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| Keywords: | Bougainvillea spp Genetic diversity Isozyme Peroxidase(POD) Esterase(EST) Clustering analysis |
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