| RT—PCR检测南方菜豆花叶病毒 |
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| 引用本文： | 李尉民 Hull.,R. RT—PCR检测南方菜豆花叶病毒[J]. 中国进出境动植检, 1997, 0(1): 28-30 |
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| 作者姓名： | 李尉民 Hull. R |
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| 作者单位： | 农业部植物检疫实验所，福建农业大学 |
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| 摘 要： | 根据南方菜豆花叶病毒（ＳＢＭＶ）两株系Ｂ和Ｃ的核酸序列设计两对引物，利用ＲＴ－ＰＣＲ可以特异地区分纯化的和０．２ｇ病叶中的两株系，ＲＴ－ＰＣＲ检测ＳＢＭＶ－Ｂ的灵敏度为１０ｐｇ。
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| 关 键 词： | 南方菜豆花叶病毒；检测；RT－PCR |
DETECTION OF SOUTHERN BEAN MOSAIC VIRUS(SBMV)BY RT-PCR |
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| Li Weimin Roger Hull Zhang Chengliang Xie Lianhui .. DETECTION OF SOUTHERN BEAN MOSAIC VIRUS(SBMV)BY RT-PCR[J]. , 1997, 0(1): 28-30 |
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| Authors: | Li Weimin Roger Hull Zhang Chengliang Xie Lianhui . |
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| Affiliation: | Li Weimin1 Roger Hull2 Zhang Chengliang1 Xie Lianhui3 1. |
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| Abstract: | Acording to the nucleotide sequences of Southern Bean Mosaic Virus(SBMV),two pairs of primers were designed.Strains B and C of SBMV extracted from 0.2g infected leaves could be differetiated by RT-PCR.The sensitivity of RT-PCR for detecting SBMV-B Virus was 10pg. |
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| Keywords: | Southern Bean Mosaic Virus Detection RT-PCR |
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