A Mycoplasma gallisepticum strain-specific DNA probe |
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Authors: | M I Khan B C Kirkpatrick R Yamamoto |
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Affiliation: | Department of Epidemiology & Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616. |
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Abstract: | Total DNA from the vaccine F strain (K810) and the reference S6-strain of Mycoplasma gallisepticum (MG) was cloned in Escherichia coli using the plasmid pUC8. A 6-kilobase fragment, specific for the vaccine strain, was identified by colony dot and Southern hybridization analyses. When labeled and used as a probe, this fragment hybridized with the homologous and one other vaccine F-strain (F2F10), but it did not hybridize with other MG strains (Fg38, S6, A5969, V503) or with three other species of avian mycoplasmas. |
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