2个小麦品种抗条锈基因遗传分析

采用常规杂交方法，以重要小麦条锈菌鉴别寄主Heines kolben和Struba Dickkopf与冬性小麦感病品种铭贤169杂交、自交和回交，获F1、F2和BC1代种子，根据条锈菌系的毒性谱，选用2E16单孢菌系，在铭贤169上繁殖。苗期抗性鉴定在人工控制的环境中进行。当麦苗第一片叶全展大约7cm时，用扫抹法接种，置于（9±2）℃接种间内黑暗保湿24h后转入低温温室内（温度为昼15~19℃，夜10~14℃）潜育发病，待感病品种铭贤169充分发病时调查侵染型，苗期抗性鉴定，并进行卡方检验，结果表明：供试品种 Heines kolben对条锈菌生理小种2E16的抗性由两对隐性互补基因控制；Struba Dickkopf对小种2E16的抗性由一对显性基因控制。

Genetic Analysis of Two Wheat Differential Hosts for Resistance to Yellow Rust

According to Puccinia striiformis west isolates virulence spectrum, race 2E16 is used for inoculation when the first leaf is well developed and about 7 cm. in length. The ‘sweep- dusting’ method of inoculation is adopted, giving a very uniform deposit of spores, after that left it in inoculation room act as humidity chambers at (9±2)℃ for 24h and then moved to low temperature greenhouse(day time for 15~19℃，night for 10~14℃) which contribute to the race infection. When the susceptible cultivar Mingxian169 are fully expressed, infection types are investigated and the resistance and susceptible phenotypes of seedlings are evaluated, Chi-square tests are employed to analyze the inheritance of differential host. The result shows that using an assessment of the wheat stripe rust reaction of seedling plants in the F1, F2 and BC1 population of a set of crosses by conventional method between wheat differential host Heines Kolben and winter wheat cultivar Mingxian169, it shows that the proportions of resistant plants obtained following inoculation of F2 populations with race 2E16 indicate that two recessive genes complementarily determine the reactions of Heines Kolben at normal temperature, but for the different crosses between wheat differential host Struba Dickkopf and winter wheat cultivar Mingxian169, it shows that the proportions of resistant plants obtained following inoculation of F2 populations with race 2E16 indicate that single dominant genes determine the reactions of Struba Dickkopf.