| 碳酸盐胁迫对偃伏梾木活性氧产生和抗氧化酶系统的影响 |
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| 引用本文： | 徐静,王庆成,孙晶,刘强. 碳酸盐胁迫对偃伏梾木活性氧产生和抗氧化酶系统的影响[J]. 东北林业大学学报, 2009, 37(9) |
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| 作者姓名： | 徐静 王庆成 孙晶 刘强 |
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| 作者单位： | 东北林业大学,哈尔滨,150040 |
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| 基金项目： | 黑龙江省重点科技计划项目，黑龙江省大庆市高新区创新基金项目 |
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| 摘 要： | 为评价偃伏梾木(Cornus stolonifera Michx.)的耐盐性,采用盆栽方法对1年生苗木在不同质量分数的NaHCO3(1.0、2.0、4.0g/kg沙土)的胁迫(7,14、21d)下叶片活性氧产生和清除系统的变化和膜质过氧化过程进行了研究.结果表明,短期胁迫时,偃伏梾木在各盐处理下叶片O-.2产生速率和H2O2的含量与对照无显著差异;CAT和APX酶活性大部分显著升高(P<0.05),分别达到对照的1.8～3.0、1.0～2.0倍;SOD活性无显著变化;丙二醛、电解质外渗率与对照无显著差异.中期胁迫叶片O-.2产生速率和H2O2的含量分别达到对照的1.9～2.9和1.2～1.5倍(P<0.05);SOD、CAT和APX酶活性均显著增加(P<0.05),SOD和CAT在中盐处理下达到最高,APX在高盐处理下达到最高;MDA含量、电解质外渗率在中低盐处理下与对照无显著差异,高盐胁迫下分别达到对照的3.0和1.4倍(P<0.05).长期胁迫叶片O-.2产生速率和H2O2的含量为对照的1.3～1.6和1.3～1.9倍(P<0.05);SOD活性在高盐处理下降低,接近对照,CAT比对照降低38%(P<0.05),APX酶仍保持较高活性(P<0.05);MDA含量、电解质外渗率分别是对照的1.3～4.7和1.8～2.8倍(P<0.05).偃伏梾木对低于2.0g/kg沙土的碳酸盐胁迫有一定的适应性.
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| 关 键 词： | NaHCO3胁迫 偃伏梾木 活性氧 抗氧化酶 |
Effect of Soil NaHCO_3 Stress on Production of Reactive Oxygen Species and Activity of Antioxidant Enzymes in Leaves of Cornus stolonifera Seedlings |
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| Xu Jing,Wang Qingcheng,Sun Jing,Liu Qiang. Effect of Soil NaHCO_3 Stress on Production of Reactive Oxygen Species and Activity of Antioxidant Enzymes in Leaves of Cornus stolonifera Seedlings[J]. Journal of Northeast Forestry University, 2009, 37(9) |
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| Authors: | Xu Jing Wang Qingcheng Sun Jing Liu Qiang |
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| Affiliation: | Xu Jing,Wang Qingcheng,Sun Jing,Liu Qiang(College of Forestry,Northeast Forestry University,Harbin 150040,P.R.China) |
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| Abstract: | The production and elimination of reactive oxygen species(ROS)and malondialdehyde(MDA),electrolytic leakage in leaves of 1-year-old Cornus stolonifera seedlings under different NaHCO3 stresses(0,1.0,2.0,and 4.0 g/kg dry soil)and different phases(7 d,14 d and 21 d)were measured to test the tolerance of C.stolonifera to soil salinity stress.No significant difference was detected in O-.2 production rate and H2O2 content in leaves between all the salt concentration treatments and the control after 7 days of str... |
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| Keywords: | NaHCO3 stress Cornus stolonifera Reactive oxygen species(ROS) Antioxidant enzymes |
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