Changes in Content of Glucosinolates and its Accumulation in Siliquae and Seeds of Oilrape (Brassica napus L.)

Rape plants were labelled by applying (NH⁴)₂³⁵SO₄ to soil. Changes in content of ³⁵S in various constituents in pods and grains were determined during siliquae development to exploit formation and accumulation of glucosinolates in oilseed rape. Content of ³⁵S in glucosinolates expressed as μmol S/g.d.w. and its relative amounts in extractable forms in young siliquae were in constant level within one week after flowering, but either absolute content or relative content of ³⁵S in glucosinolates increased largely by two weeks after flowering, thereafter the distribution of extractable ³⁵S in glucosinolates of siliquae and grains increased linearly as proceeding of its development, ³⁵S in extracts of grains almost was in form of glucosinolates after 8 weeks from flowering. Amounts of both ³⁵S in glucosinolates and dry matter per pod increased linearly with time after flowering. According to the changes of amounts of ³⁵S in other constituents per pod, it could be supposed that glucosinolates accumulated in seeds might be transported from other organs together with nutrients.