桃叶片再生不定芽的研究

以桃栽培品种曙光、金童5号和甜桃王试管苗叶片为外植体,研究了基本培养基、植物生长调节剂种类及质量浓度组合、基因型和试管苗继代次数等因素对叶片再生的影响。结果表明,适宜暗培养的培养基为LP+1.5mg·L-1TDZ+0.15mg·L-1NAA;光培养基为LP+0.5mg·L-1TDZ+0.3mg·L-1KT+0.3mg·L-1NAA和LP+0.6mg·L-1TDZ+0.3mg·L-1KT+0.4mg·L-12,4-D;金童5号和曙光具有较强的再生能力,再生率分别为21.8%和14.5%;曙光和金童5号试管苗继代第1～11次叶片的再生能力与继代前3次愈伤组织相比形成率较高;曙光再生苗在培养基1/2MS+0.5mg·L-1NAA上生根率为100%,平均生根条数为7.9。

Adventitious bud regeneration in vitro from leaves of peach cultivars

Experiment was conducted with Shuguang,Babygold 5 and Tiantaowang peach cultivars for studying the effects of the basic media,different plant growth regulators,different genotypes and subculture times on the regeneration of leaves in vitro.Among the 3 cultivars,Babygold 5 and Shuguang showed strong regeneration capacity.Results showed that the suitable basic medium and plant growth regulator was LP+1.5 mg·L-1 TDZ+0.15 mg·L-1 NAA in dark cultured period.After 3 weeks of light treatment Babygold 5 and Shuguang cultured on LP+0.5 mg·L-1 TDZ+0.3 mg·L-1 KT+0.3 mg·L-1 NAA and LP+0.6 mg· L-1 TDZ+0.3 mg·L-1 KT+0.4 mg·L-1 2,4-D grew thick buds,their regeneration frequency were 21.5% and 14.6%.The leaves of Shuguang and Babygold 5 collected from the 1st to 11th regeneration produced much higher rate of callus than those of the former 3 generations of embryos.The rooting rate of the Shuguang regeneration was 100% and the mean number of roots was 7.9 in the medium of 1/2MS +0.5 mg·L-1 NAA.