| 硝酸银对玉米幼胚组织培养的影响 |
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| 引用本文： | 杜何为,许先凤,黄敏,赵启桂,张祖新.硝酸银对玉米幼胚组织培养的影响[J].河北农业科学,2008,12(8):62-63. |
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| 作者姓名： | 杜何为 许先凤 黄敏 赵启桂 张祖新 |
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| 作者单位： | 1. 长江大学生命科学学院,湖北荆州,434025
2. 河北农业大学农学院,河北保定,071001 |
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| 基金项目： | 湖北省自然基金
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湖北省教育厅项目 |
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| 摘 要： | 以玉米自交系A188的幼胚为材料，以N6为基本培养基，在诱导、继代培养基中分别添加2、4、6、8、10、12和14mg／LAgNO3，研究了硝酸银浓度对玉米幼胚组织培养的影响。结果发现：10mg／LAgNO3有利于玉米幼胚愈伤组织的诱导，诱导率高达到93．3％；愈伤组织的继代时期，添加AgNO3反而抑制愈伤组织的增殖；10mg／LAgNO3有利于胚性愈伤组织的形成，该愈伤组织的分化率也较高。
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| 关 键 词： | 玉米 组织培养 愈伤组织 AGNO3 |
Effect of AgNO3 on Tissue Culture of A188 Immature Embryos |
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| DU He-wei,XU Xian-feng,HUANG Min,ZHAO Qi-gui,ZHANG Zu-xin.Effect of AgNO3 on Tissue Culture of A188 Immature Embryos[J].Journal of Hebei Agricultural Sciences,2008,12(8):62-63. |
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| Authors: | DU He-wei XU Xian-feng HUANG Min ZHAO Qi-gui ZHANG Zu-xin |
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| Institution: | DU He-wei1,XU Xian-feng2,HUANG Min1,ZHAO Qi-gui1,ZHANG Zu-xin2 |
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| Abstract: | Taking the A188 immature embryo as material,2,4,6,8,10,12 and 14 mg/L AgNO3 were added into induction medium and maintenance medium,respectively.The effect of AgNo3 on tissus culture of A188 immature embryos were studied.The results showed that the induction rate of callus reached 93.3% when the concentration of AgNO3 was 10 mg/L.Callus growth could be inhibited by AgNO3 during callus maintenance.The solution of AgNO3 with the concentration 10 mg/L could increase the induction rate of callus differentiation. |
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| Keywords: | Maize Tissue culture Callus AgNO3 |
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