| Screening Methods for Waterlogging Tolerance at Maize （Zea mays L.） Seedling Stage |
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| 引用本文： | LIU,Yong-zhong TANG,Bin ZHENG,Yong-lian MA,Ke-jun XU,Shang-zhong QIU,Fa-zhan. Screening Methods for Waterlogging Tolerance at Maize （Zea mays L.） Seedling Stage[J]. 中国农业科学(英文版), 2010, 9(3): 362-369. DOI: 10.1016/S1671-2927(09)60105-X |
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| 作者姓名： | LIU Yong-zhong TANG Bin ZHENG Yong-lian MA Ke-jun XU Shang-zhong QIU Fa-zhan |
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| 作者单位： | National Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University,Wuhan 430070,P.R.China |
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| 基金项目： | Acknowledgements This research was supported by the Natural Science Foundation of Hubei Province, China （2008CDB079）,and the National High Technology Research and Development Program of China （863 Program, 2006AA100103）. |
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| 摘 要： | Waterlogging strongly affects agronomic performance of maize （Zea mays L.）. In order to investigate the suitable selection criteria of waterflooding tolerant genotypes, and identify the most susceptible stage and the best continuous treatment time to waterlogging, 20 common maize inbred lines were subjected to successive artificial waterflooding at seedling stage, and waterlogging tolerance coefficient （WTC） was used to screen waterflooding tolerant genotypes. In addition, peroxidase （POD） activities and malondialdehyde （MDA） contents were measured for 6 of 20 lines. The results showed that the second leaf stage （V2） was the most susceptible stage, and 6 d after waterflooding was the best continuous treatment time. Dry weight （DW） of both shoots and roots of all lines were significantly reduced at 6 d time-point of waterlogging, compared to control. POD activities and MDA contents were negatively and significantly correlated, and the correlation coefficient was -0.9686 （P 〈 0.0001）. According to the results, WTC of shoot DW can be used for practical screening as a suitable index, which is significantly different from control and waterlogged plants happened 6 d earlier. Furthermore, leaf chlorosis, MDA content and POD activities could also be used as reference index for material screening. The implications of the results for waterlogging-tolerant material screening and waterlogging-tolerant breeding have been discussed in maize.
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| 关 键 词： | 玉米自交系 筛选方法 耐湿性 POD活性 MDA含量 基因型选择 幼苗 农艺性状表现 |
| 收稿时间： | 2009-06-09 |
Screening Methods for Waterlogging Tolerance at Maize (Zea mays L.)Seedling Stage |
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| LIU Yong-zhong,TANG Bin,ZHENG Yong-lian,MA Ke-jun,XU Shang-zhong,QIU Fa-zhan. Screening Methods for Waterlogging Tolerance at Maize (Zea mays L.)Seedling Stage[J]. 《Agricultural Sciences in China》, 2010, 9(3): 362-369. DOI: 10.1016/S1671-2927(09)60105-X |
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| Authors: | LIU Yong-zhong TANG Bin ZHENG Yong-lian MA Ke-jun XU Shang-zhong QIU Fa-zhan |
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| Affiliation: | National Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University,Wuhan 430070,P.R.China |
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| Abstract: | Waterlogging strongly affects agronomic performance of maize (Zea mays L.). In order to investigate the suitable selection criteria of waterflooding tolerant genotypes,and identify the most susceptible stage and the best continuous treatment time to waterlogging,20 common maize inbred lines were subjected to successive artificial waterflooding at seedling stage,and waterlogging tolerance coefficient (WTC) was used to screen waterflooding tolerant genotypes. In addition,peroxidase (POD) activities and malondialdehyde (MDA) contents were measured for 6 of 20 lines. The results showed that the second leaf stage (V2) was the most susceptible stage,and 6 d after waterflooding was the best continuous treatment time. Dry weight (DW) of both shoots and roots of all lines were significantly reduced at 6 d time-point of waterlogging,compared to control. POD activities and MDA contents were negatively and significantly correlated,and the correlation coefficient was -0.9686 (P<0.0001). According to the results,WTC of shoot DW can be used for practical screening as a suitable index,which is significantly different from control and waterlogged plants happened 6 d earlier.Furthermore,leaf chlorosis,MDA content and POD activities could also be used as reference index for material screening.The implications of the results for waterlogging-tolerant material screening and waterlogging-tolerant breeding have been discussed in maize. |
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| Keywords: | maize (Zea mays L.) waterlogging tolerance screening method selection criteria |
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