Contributions from different microbial processes to N2O emission from soil under different moisture regimes

Nitrous oxide emissions from a sandy-loam textured soil wetted to matric potentials of either-1.0 or-0.1 kPa were determined in laboratory experiments in which the soil was incubated in air (control), air plus 10 Pa C₂H₂ (to inhibit nitrification), 100 kPa O₂ (to suppress denitrification), 10 kPa C₂H₂ (to inhibit N₂O reduction to N₂ in denitrification) or following autoclaving. The total N₂O production, consumption and net N₂O emission from the soils together with the contributions to N₂O emission from different processes of N₂O production were estimated. The rate of N₂O production was significantly greater in the wetter soil (282 pmol N₂O g^-1 soil h^-1) than in the drier soil (192 pmol N₂O g^-1 soil h^-1), but because N₂O consumption by denitrifiers was also greater in the wetter soil, the net N₂O emissions from the wetter and the drier soils did not differ significantly. Non-biological sources made no significant contribution to N₂O emission under either moisture regime and biological processes other than denitrification and nitrification made only a small contribution (1% of the total N₂O production) in the wetter soil. Denitrifying nitrifiers were the predominant source of N₂O emitted from the drier soil and other (non-nitrifying) denitrifiers were the predominant source of N₂O emitted from the wetter soil.