| 番茄碱的高效液相色谱分析 |
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| 引用本文： | 陶永霞,段继华.番茄碱的高效液相色谱分析[J].农产品加工．学刊,2007(6):84-85. |
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| 作者姓名： | 陶永霞 段继华 |
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| 作者单位： | 1. 新疆农业大学,食品科学学院,新疆,乌鲁木齐,830052
2. 乌鲁木齐中亚食品研发中心,新疆,乌鲁木齐,830002 |
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| 摘 要： | 以新疆优势资源里格尔87-5番茄品种为原料,通过乙醇提取,中性Al2O3柱层析法纯化番茄碱粗品,确定了HPLC分析番茄碱的色谱条件:色谱柱型为Zorbax-NH2(4.6mm×150mm),流动相:浓度0.01mol/L的Na2HPO4与乙腈体积比为30∶70,柱温为35℃,流速为1.0mL/min,检测波长为205nm。
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| 关 键 词： | 番茄碱 HPLC 生物活性 |
| 文章编号： | 1671-9646（2007）06-0084-02 |
| 修稿时间： | 2007-04-13 |
Study on Tomatine in Tomato by High Performance Liquid Chromatograhy |
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| Tao Yongxia,Duan Jihua.Study on Tomatine in Tomato by High Performance Liquid Chromatograhy[J].Nongchanpin Jlagong.Xuekan,2007(6):84-85. |
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| Authors: | Tao Yongxia Duan Jihua |
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| Institution: | 1. College of Food Science, Xinjiang Agriculture University, Urumqi, Xinjiang 830052, China; 2. Central Asia Food Research and Development Centre, Urumqi, Xinjiang 830002, China |
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| Abstract: | The text adopted the neutral Al2O3 to separate and to purificate the tomatine. The high-performance liquid chromatographic was developed for quantification of tomatine on a column Zorbax-NH2(4.6 mm×150 mm),and eluted with a mixture of 0.01 mol/L Na2HPO4/acetonitrile(30/70),The column effluent was monitored at 205 nm. Temperature was 35 ℃,flow rate is 1.0ml/min. |
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| Keywords: | HPLC |
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