| 一株蛋白酶产生菌分离及鉴定 |
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| 引用本文： | 王帅,孙冬梅. 一株蛋白酶产生菌分离及鉴定[J]. 现代农业科技, 2021, 15(15) |
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| 作者姓名： | 王帅 孙冬梅 |
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| 作者单位： | 黑龙江八一农垦大学生命科学技术学院,黑龙江八一农垦大学生命科学技术学院 |
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| 摘 要： | 蛋白酶在饲料加工行业中有广泛应用。为了获得高产蛋白酶菌株，本实验采用透明圈法进行初筛，通过Folin-酚法进行复筛，从土壤中获得了一株产蛋白酶能力较高的菌株S-3。通过生理生化反应和16S rDNA基因分析，鉴定其为贝莱斯芽孢杆菌（Bacillus velezensis）。本实验为贝莱斯芽孢杆菌应用于蛋白酶生产提供了理论依据。
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| 关 键 词： | 蛋白酶 芽孢杆菌 菌种鉴定 |
| 收稿时间： | 2021-01-14 |
| 修稿时间： | 2021-01-14 |
Isolation and identification of a protease-producing bacteria |
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| sun dongmei. Isolation and identification of a protease-producing bacteria[J]. Modern Agricultural Sciences and Technology, 2021, 15(15) |
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| Authors: | sun dongmei |
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| Affiliation: | College of Life Science and Technology, Heilongjiang Bayi Land Reclamation University |
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| Abstract: | Protease is widely used in feed processing industry. In order to obtain a high-protease-producing strain, this experiment used the transparent circle method for primary screening and the Folin-phenol method for secondary screening. A strain S-3 with high protease-producing ability was obtained from the soil. Through physiological and biochemical reactions and 16S rDNA gene analysis, it was identified as Bacillus velezensis. This experiment provides a theoretical basis for the application of Bacillus Velez in protease production. |
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| Keywords: | Protease Bacillus Strain identification |
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