膳食补充磷脂型EPA/DHA结合蓝光照射对小鼠毛发再生的影响

我国水产品加工总量大,但精深加工领域薄弱,以EPA和DHA为代表的水产品功能性脂质是水产品精深加工的重要对象。脱发源于毛干新生与脱落的失衡,研究报道甘油三酯型EPA/DHA (EPA/DHA-TG)及蓝光照射均可促进毛发再生,而磷脂型EPA/DHA (EPA/DHA-PLs)对毛发再生的作用及其与蓝光照射的相互作用尚不清晰。本研究以人工脱毛小鼠建立毛发再生模型,探讨膳食补充EPA/DHA-PLs 结合蓝光照射对毛发再生的影响。雄性昆明小鼠背部脱毛后,随机分为白光照射组、蓝光照射组、蓝光照射喂食DHA-PLs或EPA-PLs组,每组8只。测量新生毛发的长度及覆盖率,HE染色观察毛囊形态及数量,分析毛发再生相关因子的mRNA及蛋白表达水平,初步探究作用机制。结果显示,蓝光照射下，膳食补充EPA/DHA-PLs可显著增加毛发长度及覆盖率,毛发再生初期DHA-PLs的效果较优,毛发再生后期显示EPA-PLs的效果最佳,其作用机制可能与对毛囊周期的调节、血管内皮生长因子VEGF的表达升高及对成纤维细胞生长因子18 (Fgf18)的调控有关。本研究提示膳食补充EPA/DHA-PLs可增效蓝光照射对小鼠毛发再生的促进作用,为水产品功能性脂质用于维护毛发健康提供科学依据。

Effects of dietary supplementation with EPA/DHA-enriched phospholipids combined with blue light irradiation on hair regeneration in mice

Aquatic product processing industrial has a large scale in China, but the field of deep processing is weak. The functional lipids of aquatic products represented by EPA and DHA are important targets for deep processing of aquatic products. Hair loss originates from the imbalance between hair shaft regeneration and shedding. EPA/DHA-enriched triglyceride (EPA/DHA-TG) and blue light irradiation can both promote hair regeneration, however it is still unclear that the effect of EPA/DHA-enriched phospholipids (EPA/DHA-PLs) on hair regeneration and its interaction with blue light irradiation. In the present study, artificial hair removal mice were used to establish a hair regeneration model and to explore the effects of dietary supplementation of EPA/DHA-PLs combined with blue light irradiation on hair regeneration. After the back hair removal, male Kunming mice were randomly divided into four groups (n=8), including white light irradiation group, blue light irradiation group, and blue light irradiation combined with dietary supplementation of DHA-PLs or EPA-PLs group. The length and coverage of regenerating hair were measured, and HE staining was applied to observe the morphology and number of hair follicles. The mRNA and protein expression levels of genes related to hair regeneration were analyzed to preliminarily explore the possible underlying mechanism. The results showed that with the blue light irradiation, dietary supplementation of EPA/DHA-PLs could significantly increase hair length and coverage. Specially, the effect of DHA-PLs was better than EPA-PLs in the early stage of hair regeneration, and the effect of EPA-PLs is more prominent compared with DHA-PLs in the later stage, which might be related to the regulation of hair follicle cycle, the increased expression of vascular endothelial growth factor (VEGF), and the regulation of fibroblast growth factor 18 (Fgf18). This study suggested that dietary supplementation of EPA/DHA-PLs could enhance the promotion of blue light irradiation on mouse hair regeneration, which provide a scientific basis for the use of aquatic functional lipids in the health maintenance of hair.