| 丝素膜的结构测定和分析 |
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| 引用本文： | 黄晨,盛家镛.丝素膜的结构测定和分析[J].蚕业科学,1996,22(3):183-186. |
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| 作者姓名： | 黄晨 盛家镛 |
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| 作者单位： | [1]安徽农业大学蚕桑系 [2]苏州丝绸工学院丝绸系 |
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| 摘 要： | 对脱胶丝素纤维、初生丝素蛋白膜、固定化ＧＯＤ－丝素膜结构进行了红外光谱分析。三者红外光谱图差异明显，丝素纤维为典型的丝Ⅱ（双向平行的β片层）结构，初生丝素膜的红外光谱显示无规卷曲或丝Ⅰ构型的特征，经戊二醛活化处理后制备的酶膜，在７００ｃｍ－１处有一显著吸收峰，表明其构型向β型转变，丝素膜的水溶率变化也证实了红外光谱显示的结果。
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| 关 键 词： | 丝素，酶膜，红外光谱，β结构 |
DETERMINATION AND ANALYSIS ON THE STRUCTURE OF BOMBYX MORI SILK FIBROIN MEMBRANES |
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| Huang Chen,Xu Xinyan.DETERMINATION AND ANALYSIS ON THE STRUCTURE OF BOMBYX MORI SILK FIBROIN MEMBRANES[J].Acta Sericologica Sinica,1996,22(3):183-186. |
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| Authors: | Huang Chen Xu Xinyan |
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| Abstract: | The structural characteristics of silk fiber,fresh silk fibroin membrane and GOD-silk fibroin membrane were investigated by means of infrared(IR)spectrum respective-ly.IR spectra indicated that silk fibre remained typical anti-parallel β-sheet(silkⅡ)form and,the freshsilk membrane had the Characteristics of the random coil structure.However,increasing of absorption frequencies of the GOD-membrane induced by glu-taraldehyde(GA)solution at 700cm-1,which was assigned to amide Ⅳ bands of silk Ⅱform,showed the structure of the enzyme-membrane transformed from random coil toanti-parallel β-sheet form(silkⅡ).Decreasing of the water solubility of the enzyme-membrane confirmed the results showed by IR spectra. |
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| Keywords: | Fibroin Enzyme membrane IR spectrumβstructure |
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