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| 土槿皮乙酸B通过活性氧和有丝分裂原蛋白激酶诱导MCF-7细胞凋亡的研究 | |
| 引用本文： | 刘春禹,郑桂彬,于静华.土槿皮乙酸B通过活性氧和有丝分裂原蛋白激酶诱导MCF-7细胞凋亡的研究[J].中国畜牧兽医,2013,40(3):146-148. |
| 作者姓名： | 刘春禹 郑桂彬 于静华 |
| 作者单位： | 1. 吉林省中医院针灸科,吉林长春,130000 2. 吉林大学白求恩第一医院甲状腺外科,吉林长春,130000 3. 吉林大学白求恩第一医院艾滋病与病毒研究所,吉林长春,130000 |
| 基金项目： | 吉林省中医药管理局，吉林省中医药管理局 |
| 摘 要： | 土槿皮乙酸B(PAB)通过活性氧诱导肿瘤细胞MCF-7凋亡,同时也通过调控有丝分裂原蛋白激酶(MAPK)家族蛋白来调控凋亡,但活性氧和MAPK的关系还不清楚,本研究探讨活性氧和MAPK家族蛋白的关系。4 μmol/L PAB在36 h诱导细胞死亡,活性氧清除剂抑制PAB的作用,并降低PAB的抑制率;同时,PAB促进JNK磷酸化,抑制ERK的磷酸化,此作用被活性氧清除剂抑制。 |
| 关 键 词： | 土槿皮乙酸B 活性氧 凋亡 MAPK家族蛋白 人乳腺癌MCF-7细胞 |
| 收稿时间： | 2012-12-19 |
Study of Pseudolaric Acid B Induced Apoptosis through ROS Regulating MAPK in MCF-7 Cells |
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| LIU Chun-yu , ZHENG Gui-bin , YU Jing-hua.Study of Pseudolaric Acid B Induced Apoptosis through ROS Regulating MAPK in MCF-7 Cells[J].China Animal Husbandry & Veterinary Medicine,2013,40(3):146-148. | |
| Authors: | LIU Chun-yu ZHENG Gui-bin YU Jing-hua |
| Institution: | 1. Acupunture Department, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun 130000, China;2. Department of Thyroid Surgery, The First Hospital of Jilin University, Changchun 130000, China;3. Research Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun 130000, China |
| Abstract: | The assay was aimed to study the relationship of ROS and MAPK family protein in pseudolaric acid B (PAB)-induced MCF-7 cell apoptosis. Contrast phase microscope was used to observe cellular morphologic alternation; MTT analysis detected the inhibitory ratio; flow cytometric analysis of DCFH-DA staining determined the level of ROS, and Western blotting detected the protein expression. We found that PAB induced MCF-7 cell apoptosis through increasing ROS and increased the phosphoration of JNK and decrease the phosphoration of ERK. |
| Keywords: | pseudolaric acid B ROS apoptosis MAPK MCF-7 family protein |
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