不同硒浓度日粮对小鼠肝脏和睾丸组织中部分硒蛋白mRNA水平的影响

选取80只7周龄雄性BALB/c小鼠,随机分为Ⅰ、Ⅱ、Ⅲ、Ⅳ组,每组20只,使Ⅰ、Ⅱ、Ⅲ、Ⅳ组基础日粮中硒含量达到0.045、0.1、0.4和0.8 mg/kg。结果表明:①Ⅰ组能显著降低小鼠肝脏中硒的沉积量(P<0.05);②Ⅰ组谷胱甘肽过氧化物酶1(glutathione peroxidase 1,GPx1)的mRNA相对表达量明显低于Ⅱ、Ⅲ组(P<0.05),且Ⅱ、Ⅲ组在肝脏中差异显著(P<0.05),在睾丸中差异不显著(P>0.05);③Ⅰ、Ⅲ组肝脏中谷胱甘肽过氧化物酶4(glutathione peroxidase 4,GPx4)的mRNA相对表达量差异不显著(P>0.05),但均显著低于Ⅱ组(P<0.05),而各试验组间在睾丸中差异均不显著(P>0.05);④随着日粮中硒添加量的增加,肝脏和睾丸中硫氧还蛋白还原酶2(thioredoxin reductase 2,TrxR2)的mRNA相对表达量显著提高(P<0.05)。因此,在肝脏和睾丸组织中,饲喂低硒饲料能显著降低硒的沉积量和GPx1、GPx4、TrxR2的mRNA相对表达量(P<0.05);而饲喂高硒饲料能显著降低GPx1的mRNA相对表达量(P<0.05),同时显著增加TrxR2的mRNA相对表达量(P<0.05)。提示,硒对GPx1、GPx4和TrxR2 mRNA相对表达量的调节存在组织差异性。

Effect of Different Dietary Selenium Concentration on mRNA Levels of Some Selenoprotein in Mice Liver and Testis

A total of 80 7-week-old male BALB/c mice were randomly divided into four groups (Ⅰ,Ⅱ,Ⅲ and Ⅳ groups),and 20 mice in each group. Each group was fed with the diet containing 0.045,0.1,0.4,and 0.8 mg/kg Se,respectively. The results showed that the content Se of Ⅰ group in the liver reduced significantly (P<0.05). Glutathione peroxidase 1 (GPx1)mRNA levels of Ⅰ group was significant lower than Ⅱ and Ⅲ groups (P<0.05),while Ⅱ and Ⅲ groups were significant differences in the liver (P<0.05),and no significant differences in testis (P>0.05). Glutathione peroxidase 4 (GPx4) mRNA level of Ⅰand Ⅲ groups in liver had no significant difference (P>0.05),but were significant lower than group Ⅱ (P<0.05),while each group had no difference in the testis (P>0.05). Relative mRNA expression of thioredoxin reductase 2 (TrxR2) in liver and testis improved significantly (P<0.05) with the increasing of dietary Se supplemention. In conclusion,in liver and testis,deficient Se dietary could significantly reduce selenium deposition and relative mRNA expression of GPx1,GPx4 and TrxR2,however,excessive Se dietary could significantly reduce GPx1 mRNA levels (P<0.05) and significantly increase TrxR2 mRNA levels (P<0.05),it showed that there was difference for different organs.