| 不同样品中犬输血传播性病毒的PCR检测比较与基因分析 |
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| 引用本文： | 郁达义,叶剑波,袁聪俐,华修国. 不同样品中犬输血传播性病毒的PCR检测比较与基因分析[J]. 中国畜牧兽医, 2013, 40(3): 59-61 |
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| 作者姓名： | 郁达义 叶剑波 袁聪俐 华修国 |
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| 作者单位： | 1. 闵行区动物疾病预防控制中心,上海 201109;2. 上海交通大学农业与生物学院, 上海市兽医生物技术重点实验室,上海 200240 |
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| 摘 要： | 根据NCBI上输血传播性病毒(Torque-Teno virus,TTV)犬基因组序列(Cf-TTV10,GenBank登录号:AB076002)设计嵌套引物,从犬血清与粪便中鉴定出TTV。199份犬血清样品中检出阳性率为14.6%(29/199),158份犬粪便样品的检出率为12.7%(20/158),通过基因分析与鉴定确定为同一型,验证了从粪便样品和血液样品的提取检测的犬TTV阳性率基本保持一致(P>0.05),为下一步进行TTV的传染特性和分子流行病学的研究提供了基础。
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| 关 键 词： | 犬输血传播性病毒 PCR检测 基因分析 |
| 收稿时间： | 2012-08-29 |
The PCR Detection and Gene Analysis of Torque-Teno Canis Virus in Different Extraction Samples |
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| YU Da-yi , YE Jian-bo , YUAN Cong-li , HUA Xiu-guo. The PCR Detection and Gene Analysis of Torque-Teno Canis Virus in Different Extraction Samples[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(3): 59-61 |
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| Authors: | YU Da-yi YE Jian-bo YUAN Cong-li HUA Xiu-guo |
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| Affiliation: | 1. Animal Disease Control Center of Minhang District, Shanghai 201109, China;2. Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, ShanghaiJiao tong University, Shanghai 200240, China |
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| Abstract: | Based on the sequence of Torque-Teno canis virus registered on NCBI (Cf-TTV10, accession No.AB076002), we designed primers for nested PCR to detect the Torque-Teno canis virus in serum and fecal samples. It was showed that there were 14.6% Torque-Teno canis virus positive canine serum samples and 12.7% positive fecal samples, and it was verified that the gene type was same. There was no significant difference in different extraction samples(P>0.05). This research provided the basis for the further study of TTV characteristics and molecular epidemiology. |
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| Keywords: | Torque-Teno canis virus PCR detection gene analysis |
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