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| Genetic Relationships among Prunus mume var. pendula Using AFLP Markers | |
| 作者单位： | Ming Jun1* Zhang Qixiang2 Ru Guangxin3 Mao Qingshan4 Yan Xiaolan4 Lan Yanping5 1Department of Horticulture，Hubei Agricultural College，Jingzhou 434025，P. R. China 2College of Landscape Architecture，Beijing Forestry University，Beijing 100083，P. R. China 3Department of Forestry，Henan Agricultural University，Zhengzhou 450000，P. R. China 4The Research Center of China Mei Flowers，Wuhan 430000，P. R. China 5Institute of Plant Nutrition and Resource，Beijing Academy of Agriculture and Forestry，Beijing 100091，P. R. China |
| 基金项目： | the Natural Science Foundation of Hubei Province，P. R. China (Grant No. 2001ABB118) |
| 摘 要： | Genetic relationships among Prunus mume var. pendula were studied by using AFLP markers. 18 accessions representing 14 cultivars ofPrunus murne var. pendula were selected from the germplasm collection at the Research Center of China Mci Flower. Seven Mse I-EcoR I AFLP primer combinations revealed 450 legible bands, and 269 of which were polymorphic markers. A similarity matrix was prepared using the simple matching coefficient of similarity and Nei‘s (72) distance coefficient. A UPGMA dendrogram demonstrated the genetic relationships of the cultivars. The information given by AFLP markers was basically consistent with the morphological classification and the evolutionary history of the morphotypes, and roughly supported the new revised classification system for Chinese Mci Cultivars. But there were still several exceptions: 1) the ‘Guhong Chuizhi‘ inserted between the ‘Tiaoxue Chuizhi‘ and the ‘Danfen Chuizhi‘; 2) the ‘Wufu Chuizhi‘ kept off the Pink Pendant Form, and the ‘Moshan Chuizhi‘ was removed from Viridiflora Pendant Form; 3) the ‘Danbi Chuizhi‘ and the ‘Shuangbi Chuizhi‘ of Viridiflora Pendant Form got together well but fell within the Pink Pendant Form. |
Genetic Relationships among Prunus mume var. pendula Using AFLP Markers |
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| MING Jun,Zhang Qixiang,Ru Guangxin,Mao Qingshan,Yan Xiaolan,Lan Yanping. Genetic Relationships among Prunus mume var. pendula Using AFLP Markers[J]. Forestry Studies in China, 2003, 5(4) | |
| Authors: | MING Jun Zhang Qixiang Ru Guangxin Mao Qingshan Yan Xiaolan Lan Yanping |
| Affiliation: | 1. Department of Horticulture, Hubei Agricultural College, Jingzhou 434025, P. R. China 2. College of Landscape Architecture, Beijing Forestry University, Beijing 100083, P. R. China 3. Department of Forestry, Henan Agricultural University, Zhengzhou 450000, P. R. China 4. The Research Center of China Mci Flowers, Wuhan 430000, P. R. China 5. Institute of Plant Nutrition and Resource, Beijing Academy of Agriculture and Forestry, Beijing 100091, P. R. China |
| Abstract: | Genetic relationships among Prunus mume var. pendula were studied by using AFLP markers. 18 accessions representing 14 cultivars of Prunus mume var. pendula were selected from the germplasm collection at the Research Center of China Mei Flower. Seven Mse I-EcoR I AFLP primer combinations revealed 450 legible bands, and 269 of which were polymorphic markers. A similarity matrix was prepared using the simple matching coefficient of similarity and Neis (72) distance coefficient. A UPGMA dendrogram demonstrated the genetic relationships of the cultivars. The information given by AFLP markers was basically consistent with the morphological classification and the evolutionary history of the morphotypes, and roughly supported the new revised classification system for Chinese Mei Cultivars. But there were still several exceptions: 1) the Guhong Chuizhi inserted between the Tiaoxue Chuizhi and the Danfen Chuizhi; 2) the Wufu Chuizhi kept off the Pink Pendant Form, and the Moshan Chuizhi was removed from Viridiflora Pendant Form; 3) the Danbi Chuizhi and the Shuangbi Chuizhi of Viridiflora Pendant Form got together well but fell within the Pink Pendant Form. |
| Keywords: | Prunus mume Sieb. et Zucc. amplified fragment length polymorphism (AFLP) Prunus murne var. pendula (Pendulous Mci Group) genetic relationship |
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