| 生药防风不定芽分化影响因素的研究 |
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| 引用本文： | 韩珊珊,贝丽霞,陈祥梅. 生药防风不定芽分化影响因素的研究[J]. 黑龙江八一农垦大学学报, 2010, 22(4): 15-18,22 |
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| 作者姓名： | 韩珊珊 贝丽霞 陈祥梅 |
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| 作者单位： | 1. 黑龙江八一农垦大学农学院,大庆,163319
2. 淄博市国家高新技术产业开发区招商局 |
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| 基金项目： | 黑龙江省研究生科研创新资金项目，大庆市科技局课题 |
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| 摘 要： | 以防风愈伤组织为材料,研究了培养基、激素组合、光照、愈伤诱导周期和继代培养次数对愈伤组织不定芽分化的影响。结果表明:适合防风愈伤组织芽分化的最佳培养基为MS+NAA0.2+KT0.2+6-BA1.0;继代培养的周期为25d,继代培养2次防风不定芽的分化率较高。随着光照强度从5001x到20001x的增加,防风愈伤组织产生的芽数增加,丛生芽健壮,光照强度20001x最适合防风愈伤分化出不定芽。
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| 关 键 词： | 防风 愈伤组织 芽分化 |
Research on Influencing Factors of Adventitious Bud Differentiation of Oficinal Foliage Sapashnikovia Divaricata |
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| Han Shanshan,Bei Lixia,Chen Xiangmei. Research on Influencing Factors of Adventitious Bud Differentiation of Oficinal Foliage Sapashnikovia Divaricata[J]. journal of heilongjiang bayi agricultural university, 2010, 22(4): 15-18,22 |
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| Authors: | Han Shanshan Bei Lixia Chen Xiangmei |
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| Affiliation: | 1.College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319;2.The China Merchants of National Hi-Tech Industrial Development Zone in Zibo) |
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| Abstract: | By using the Saposhnikovia divaricata callus as the test materials,we have researched the effects of the substrate、Hormone combination 、light,callus induction period and the number of subculture on callus adventitious bud differentiation.The result showed that the best substrate used bud polarization of culture the callus,it is MS＋NAA0.2＋KT0.2＋6-BA 1.0;the suitable callus subculture period for the Saposhnikovia divaricata was 25 d,under two subcultures,the rate of callus adventitious bud differentiation was higher.As the light intensity increased from 5 001 x to 20 001 x,the shoots produced by the Saposhnikovia divaricata callus increased,the caespitosa shoots were robust,the light intensity 20 001 x was the best condition for the Saposhnikovia divaricata calli emergence. |
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| Keywords: | saposhnikovia divaricata callusbud differentiation |
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