| 昆明香石竹斑驳病毒鉴定 |
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| 引用本文： | 蔡 红,孔宝华,吴建宇,陈海如,段永嘉.昆明香石竹斑驳病毒鉴定[J].云南农业大学学报,2001,16(4):263-266. |
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| 作者姓名： | 蔡 红 孔宝华 吴建宇 陈海如 段永嘉 |
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| 作者单位： | [1]云南农业大学，云南省植物病理重点实验室，云南昆明650201 [2]云南农业大学，云南省植物病理重点实验室，云南昆 |
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| 基金项目： | 云南省省院省校合作课题(98Y9009) |
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| 摘 要： | 从表现为叶斑驳、花碎色症状的香石竹病株上获得一病毒分离物,经电镜负染观察,该病毒为直径是28～33 nm的球状粒子,病毒提取液经紫外光测定呈典型核蛋白吸收曲线,OD260/OD280=1.70;血清学反应与CarMV抗血清出现明显的沉淀线。由此,可基本认为该病毒分离物为香石竹斑驳病毒(Carnation mottle virus, CarMV)。
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| 关 键 词： | 香石竹 香石竹斑驳病毒 鉴定 |
| 文章编号： | 1004-390X(2001)04-0263-04 |
| 收稿时间： | 2000-3-6 |
Identification of Carnation Mottle Viruson Carnation in Kunming |
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| CAI Hong,KONG Bao hua,WU Jian yu,CHEN Hai ru,DUAN Yong jia.Identification of Carnation Mottle Viruson Carnation in Kunming[J].Journal of Yunnan Agricultural University,2001,16(4):263-266. |
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| Authors: | CAI Hong KONG Bao hua WU Jian yu CHEN Hai ru DUAN Yong jia |
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| Institution: | (Phytopathology Laboratory of Yunnan Province, Y A U, Kunming 650201,China) |
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| Abstract: | A isolated virus from carnation with mosaic on leaves and breaking on flowers. Through TEM, the virus particle was spherical about 28-33 nm in diameter. The purified virus from infected tissue showed typical ultraviolet absorption of nucleoprotein, OD260/OD280=1.70. Virus particles were in bar shape with antiserum of CarMV by immunodiffsion tests. On the basis of these characteristics, the virus isolate was considered to be identical to carnation mottle virus (CarMV). |
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| Keywords: | carnation carnation mottle virus identification |
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