‘红阳’猕猴桃叶片和带芽茎段的组织培养快繁技术

以‘红阳’猕猴桃雌株的幼嫩叶片和带腋芽茎段为外植体,通过诱导叶片愈伤组织及不定芽、带腋芽茎段直接出芽,不定芽增殖、生根,从而建立高效稳定的快速繁殖体系。结果表明：叶片极易诱导形成愈伤组织,试验中各种类型培养基对其的诱导率均达100%,其中MS+1.0 mg/L ZT+0.1 mg/L NAA 所得到的愈伤组织易于分化成苗,芽分化率达到99.33%,不定芽的增殖系数平均达到5.2个；MS+1.0 mg/L 6-BA+0.1 mg/L NAA最适宜带芽茎段上腋芽的萌发,最高诱导率达83.33%,不定芽在MS+2.0 mg/L 6-BA+0.2 mg/L NAA+0.1 mg/L GA中的增殖系数平均达4.5；培养基1/2 MS+0.7 mg/L IBA诱导不定芽生根效果佳。

Tissue culture and rapid micro-propagation from leaves and stems of kiwifruit (Actinidia chinensis cv. Hongyang)

A stable and efficient rapid propagation system was established from the female Actinidia chinensis cv.Hongyang young leaves and stem segments with axillary buds in vitro. Leaves were cultured for the initiating of callus, proliferation of adventitious buds; and axillary buds were cultured for sprouting, multiplication and rooting. The results indicated that callus formation was easy and proliferation efficiency achieved 100% when explants from leaves were cultured on MS supplemented with 1.0 mg·L^-1 zeatin and 0.1 mg·L^-1 naphthylacetic acid (NAA). The average multiplication coefficient was 5.2,and the highest induction rate was 93.33%. The medium of MS supplemented with 1.0 mg·L^-1 6-benzylaminopurine (6-BA) and 0.1 mg·L^-1 NAA was optimal for sprouting of axillary bud,and the highest induction rate was 8333%. The multiplication coefficient of adventitious bud was 4.5 in the medium of MS supplemented with 2.0 mg·L^-1 6|BA, 0.2 mg·L^-1 NAA and 0.1 mg·L^-1 gibberellic acid (GA). Moreover, the medium of 1/2 MS with 0.7 mg·L^-1 indolebutyric acid (IBA) was good for rooting.