| 固体培养基上浸润培养提高花粉植株诱导率的研究 |
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| 引用本文： | 王培,陈玉蓉.固体培养基上浸润培养提高花粉植株诱导率的研究[J].华北农学报,1992,7(3):59-65. |
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| 作者姓名： | 王培 陈玉蓉 |
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| 作者单位： | 河北省农科院粮油作物研究所，河北省农科院粮油作物研究所 石家庄 050031，石家庄 050031 |
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| 摘 要： | 在30×170mm的试管内,倒入25～30ml加琼脂固化的C_(17)培养基,接种冬小麦花药后注入2ml左右不加琼脂而浓度相同的C_(17)培养液浸润培养,冬小麦花药愈伤组织诱导率可从固体培养基的13.94％提高到29.95％,愈伤组织的绿苗分化率还比固体培养基提高10.2％,分化绿苗丛数占接种花药的10.94％,是固体培养的2.37倍。
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| 关 键 词： | 小麦 花药培养 浸润 固体培养 |
A Study on Increasing Induction Frequency of Pollen Plants by Culture with a Thin Liquid Layer on Solidified Medium |
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| Wang Pei Chen Yurong.A Study on Increasing Induction Frequency of Pollen Plants by Culture with a Thin Liquid Layer on Solidified Medium[J].Acta Agriculturae Boreali-Sinica,1992,7(3):59-65. |
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| Authors: | Wang Pei Chen Yurong |
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| Abstract: | After inoculation of winter wheat anther into glass tubes containing 25 to 30 ml agar-solidified C1 7 medium, about 3 mm lipuid C1 7 medium was added. This raised the callus induction frequency of winter wheat anthes from 13.94% to 29.95% and the green plant regeneration frequency from calli by 10.20% in comparison with culture on solidified media. By this method the number of regenerated green plants accounted for 10.94% f the inoculated anthers which was 2.73 times as much as that by culture en solidified medium. |
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| Keywords: | Winter wheat Anther culture Thin liquid layer Solidified medium |
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