草地早熟禾高效丛生芽体系的建立

本试验以草地早熟禾无菌种子苗茎尖为外植体,建立了高效的草地早熟禾丛生芽离体培养体系。结果表明:茎尖在附加0.5mg/L2,4-D和3.0mg/L6-BA的MS培养基上可诱导茎尖部位基部膨大,且诱导率最高。膨大的基部在2.0mg/L6-BA的丛生芽诱导培养基上,其诱导频率最大,然后将丛生芽转入附加3.0mg/L6-BA和0.07mg/L2,4-D的培养基上可进行继代培养,丛生芽在1/2MS培养基上生根率达100%。

High Efficiency Induction of Multiple Shoots from Poa pratensis L.

In this research,an in vitro proliferation system of mutiple shoot clumps was established by using shoot apices of seedlings as explants at a high efficiency.The results showed that swelled bases were induced from shoot apices on MS medium supplemented with 0.5 mg/L 2,4-D and 3.0 mg/L 6-BA,then were transferred to shoot clumps induction medium containing 2.0 mg/L 6-BA for the formation of mutiple shoot clumps,and multiple shoot clumps were obtained on proliferation medium supplemented with 3.0 mg/L 6-BA and...