豇豆籽肽的制备及抗氧化活性研究

目的]采用酶法制备豇豆籽蛋白肽,并探讨其抗氧化活性。方法]先用碱提酸沉法制备豇豆籽蛋白,然后分别用碱性蛋白酶和木瓜蛋白酶水解制备豇豆籽肽,后者脱盐后进行DPPH自由基、羟基自由基和超氧阴离子清除活性分析。结果]碱性蛋白酶水解能力强于木瓜蛋白酶,2种酶水解后的豇豆籽肽对3种自由基均具有较好的清除活性。在相应浓度范围内,对DPPH自由基最大清除率分别为63.92%(碱性蛋白酶)和63.06%(木瓜蛋白酶),半数抑制浓度IC50分别为3.20和3.28 mg/ml;对羟基自由基最大清除率分别为87.66%(碱性蛋白酶)和85.88%(木瓜蛋白酶),相应IC50分别为4.21和4.89 mg/ml;对超氧阴离子自由基的最大清除率64.06%(碱性蛋白酶)和47.92%(木瓜蛋白酶)。结论]研究可为豇豆籽蛋白肽的深度开发和综合利用提供一定的理论基础。

Preparation Of Cowpea seed peptide and Study on Its Antioxidation

Objective ] To prepare eowpea seed protein peptide by using enzyme method and discuss its antioxidation. Method ] Prepare cowpea seed protein by alkali extraction and acid precipitation method. Then, prepare cowpea seed peptide with alkali protease and pawpaw protease, after desalinization, removal activity analysis by DPPH free radical, hydroxyl free radical and superoxide anion were carried out. Result ] The hydrolysis ability of alkali protease is stronger than pawpaw protease, cowpea seed peptide after hydrolysis all have scavenging ability on three free radicals. Within the corresponding concentration range, the maximum scavenging rate to DPPH free radicals are 63.92% （ alkali protease） and 63.06% （paw- paw protease） respectively, 1Cs0 are 3.20 and 3.28 mg/ml; the maximum scavenging rate to hydroxyl free radical are 87.66% （alkali protease） and 85.88% （pawpaw protease）, 1C5o are 4.21 and 4.89 mg/ml ; the maximum scavenging rate to uperoxide anion are 64. 06% （ alkali protease） mad 47.92% （pawpaw protease）. Conclusion ] The study provides theoretical basis for deeply development and comprehensive utilization of cowpea seed protein peptide.