甜叶菊组培快繁技术研究

[目的]研究甜叶菊的组培快繁技术。[方法]以甜叶菊茎段为外植体,筛选其最佳外植体消毒条件、最佳不定芽增殖及壮苗生根培养基。[结果]甜叶菊外植体材料的灭菌最佳条件为75%酒精20 s,0.1%升汞5～8 min;最佳不定芽增殖培养基为MS+1.0 mg/L6-BA+0.1 mg/L NAA,此时不定芽增殖率达80%;最佳壮苗生根培养基为1/2MS+0.1 mg/L 6-BA+0.1 mg/L NAA+0.2 mg/L IBA。[结论]该研究为甜叶菊的工厂化规模生产提供了科学依据。

Research on Tissue Culture and Rapid Propagation Technology of Stevia rebaudiana

[ Objective ] The aim was to study the tissue culture and rapid propagation technology of Stevia rebaudiana. [ Method ] The best ex plants sterilization method, optimal media of adventitious bud proliferation and seedling rooting were screened by using shoot tips of S. rebaudi- aria as explants. [ Result] The best explants sterilization method was to sterilized with 0.1% HgC12 for 5 - 8 min after being treated with 75% alcohol for 20 s ; tbe optimal adventitious bud proliferation medium was MS ＋ 1.0 mg/L 6-BA ＋ 0.1 mg/L NAA and the adventitious bud pro- liferation rate was 80% ; the optimal seedling rooting medium was 1/2MS ＋ 0.1 mg/L 6-BA ＋ 0.1 mg/L NAA ＋ 0.2 mg/L IBA. [ Conclusion ] This study Drovided a scientific basis for the industrialized and larze-scale production of S. rebaudiana.