| 应用SSR标记技术进行引物筛选及玉米杂交种纯度的鉴定 |
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| 引用本文： | 闫米格,骈跃斌,李素玲,原佳敏,张君捷. 应用SSR标记技术进行引物筛选及玉米杂交种纯度的鉴定[J]. 吉林农业科学, 2011, 36(3) |
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| 作者姓名： | 闫米格 骈跃斌 李素玲 原佳敏 张君捷 |
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| 作者单位： | 山西省农业科学院食用菌研究所,太原,030031 |
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| 基金项目： | 山西省农业科学院攻关项目(YYZJC1013) |
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| 摘 要： | 利用SSR标记技术分别筛选出玉米杂交种强盛16、强盛9、强盛62、强盛11的特异引物,建立了4个品种的种子纯度鉴定试剂盒。试验表明,应用此试剂盒可快速、有效地鉴定4个杂交种的种子纯度。
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| 关 键 词： | 玉米杂交种 SSR标记技术 种子纯度鉴定 引物筛选 |
Screening of Primers and Testing of Seed Purity of Maize Hybrid by SSR Marker Technique |
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| YAN Mi-ge,PIAN Yue-bin,LI Su-ling,YUAN Jia-min,ZHANG Jun-jie. Screening of Primers and Testing of Seed Purity of Maize Hybrid by SSR Marker Technique[J]. Journal of Jilin Agricultural Sciences, 2011, 36(3) |
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| Authors: | YAN Mi-ge PIAN Yue-bin LI Su-ling YUAN Jia-min ZHANG Jun-jie |
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| Affiliation: | YAN Mi-ge,PIAN Yue-bin,LI Su-ling,YUAN Jia-min,ZHANG Jun-jie(Edible Fungi Research Institute,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China) |
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| Abstract: | In this study,polymorphic SSR primer pairs were screened for maize hybrids'Qiangsheng 16','Qiangsheng 9','Qiangsheng 62','Qiangsheng 11',respectively.With SSR marker technique,the seed purity testing kits of four hybrids were established.The results showed that the testing kits could be effectively and rapidly used for seed purity analysis of four hybrids. |
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| Keywords: | Maize hybrid SSR Seed purity Screening of Primers |
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