| 猪圆环病毒2型HN0712株ORF2基因的原核表达 |
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| 引用本文： | 王菲,张志,李相钊,单虎,吴发兴,张燕霞,李晓成. 猪圆环病毒2型HN0712株ORF2基因的原核表达[J]. 动物医学进展, 2008, 29(10) |
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| 作者姓名： | 王菲 张志 李相钊 单虎 吴发兴 张燕霞 李晓成 |
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| 作者单位： | 青岛农业大学,山东,青岛,266109;中国动物卫生与流行病学中心,山东,青岛,266032;中国动物卫生与流行病学中心,山东,青岛,266032;青岛农业大学,山东,青岛,266109;青岛农业大学,山东,青岛,266109;青岛农业大学,山东,青岛,266109 |
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| 摘 要： | 根据已测猪圆环病毒2型湖南分离株（HN0712）全基因序列，设计一对克隆和表达HN0712株ORF2全基因（709bp）的引物，利用原核表达载体pET-32a（＋）获得重组pET—ORF2质粒。经IPTG诱导后成功地在BL21宿主菌中表达分子质量约为48．2ku的融合蛋白。Western blot分析表明，该融合蛋白具有较好的反应原性。
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| 关 键 词： | 猪圆环病毒2型 ORF2基因 原核表达 |
Prokaryotic Expression of HN0712 ORF2 Gene of PCV-2 |
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| WANG Fei,ZHANG Zhi,LI Xiang-zhao,SHAN Hu,WU Fa-xing,ZHANG Yan-xia,LI Xiaocheng. Prokaryotic Expression of HN0712 ORF2 Gene of PCV-2[J]. Progress In Veterinary Medicine, 2008, 29(10) |
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| Authors: | WANG Fei ZHANG Zhi LI Xiang-zhao SHAN Hu WU Fa-xing ZHANG Yan-xia LI Xiaocheng |
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| Affiliation: | 1.Qingdao Agricultural University;Qingdao;Shandong;266109;China;2.China Animal Health and Epidemiology Center;266032;China |
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| Abstract: | A pair of primers were designed according to the porcine circovirus type 2(PCV-2)sequences to amplify the complete open reading frame(ORF2) gene of PCV-2 isolation strain from Hunan province(HN0712).The amplified products were cloned into pET-32a(+) to construct recombinant plasmid pET-ORF2.After IPTG induction of different concentrations in BL21 bacteria,a 48.2 ku fusion protein was expressed and analyzed by Western blot.The fusion protein had a good immunoreactiviby with PCV-2. |
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| Keywords: | PCV-2 ORF2 prokaryotic expression |
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