| 基于HTP标记对玉米DH、RIL和BC群体遗传规律的比较分析 |
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| 引用本文： | 张云龙,马世鹏,许理文,赵怡锟,王蕊,朱少喜,霍永学,张立平,郭瑞佳,李莉,王元东,番兴明,王凤格.基于HTP标记对玉米DH、RIL和BC群体遗传规律的比较分析[J].玉米科学,2024,32(2):8-15. |
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| 作者姓名： | 张云龙 马世鹏 许理文 赵怡锟 王蕊 朱少喜 霍永学 张立平 郭瑞佳 李莉 王元东 番兴明 王凤格 |
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| 作者单位： | 北京市农林科学院玉米研究所/玉米DNA指纹及分子育种北京市重点实验室, 北京 100097;北京市农林科学院玉米研究所/玉米DNA指纹及分子育种北京市重点实验室, 北京 100097;中国农业大学农学院种子科学与技术研究中心, 北京 100193;北京市农林科学院杂交小麦研究所/杂交小麦分子遗传北京市重点实验室, 北京 100097;云南省农业科学院粮食作物研究所, 昆明 650205 |
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| 基金项目： | 北京学者计划项目(BSP041)、北京市农林科学院科技创新能力建设专项(KJCX20200102)、国家玉米产业技术体系项目(CARS-02-11) |
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| 摘 要： | 以先玉335双单倍体(Doubled Haploids,DH)群体、掖107×昌7-2重组自交系(Recombined inbred lines,RIL)群体和京724×京92回交1代(Backcross,BC)群体为试材,采用全基因组覆盖的新型区块标记——单倍型标签多态性(haplotype-tag polymorphisms,HTP)标记,从基因型分离比、亲本遗传片段和重组交换3个角度解析DH、RIL、BC群体的遗传规律。结果表明,DH群体虽然偏分离高于BC群体,平均交换频率低于RIL群体,但该群体样本中不存在杂合和外源片段,均为纯合片段;平均亲本遗传片段长度为384个HTP,高于RIL群体;单代上拥有最高的重组交换次数,相较于其他群体,在群体特点和创制成本等方面具有较多优势。
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| 关 键 词： | 玉米 DH RIL BC HTP标记 遗传规律 |
Comparative Analysis of Genetic Laws of Maize DH, RIL and BC Populations Based on HTP Markers |
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| ZHANG Yun-long,MA Shi-peng,XU Li-wen,ZHAO Yi-kun,WANG Rui,ZHU Shao-xi,HUO Yong-xue,ZHANG Li-ping,GUO Rui-ji,LI Li,WANG Yuan-dong,FAN Xing-ming,WANG Feng-ge.Comparative Analysis of Genetic Laws of Maize DH, RIL and BC Populations Based on HTP Markers[J].Journal of Maize Sciences,2024,32(2):8-15. |
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| Authors: | ZHANG Yun-long MA Shi-peng XU Li-wen ZHAO Yi-kun WANG Rui ZHU Shao-xi HUO Yong-xue ZHANG Li-ping GUO Rui-ji LI Li WANG Yuan-dong FAN Xing-ming WANG Feng-ge |
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| Institution: | Maize Research Institute, Beijing Academy of Agricultural and Forestry Science/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097;Maize Research Institute, Beijing Academy of Agricultural and Forestry Science/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097;Center of Seed Science and Technology, College of Agriculture and Biotechnology, Beijing 100193;Beijing Key Laboratory of Molecular Genetics in Hybrid Wheat, Institute of Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097;Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205, China |
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| Abstract: | In this study, HTP markers were used to analysis the genetic laws of Xianyu335 DH population,Ye107×Chang7-2 RIL population and Jing724×Jing 92 population, and Jing724×Jing 92 BC1 population from three perspectives: genotype segregation ratio, parental genetic fragmentation, and recombination exchange. The reesults showed that the average exchange frequency of DH population is lower than that of the RIL population, and no heterozygous and exogenous fragments. The average parental genetic fragment length is 384 HTP higher than RIL population, and it has the highest number of recombination exchanges in a single generation. Compared to BC1 and RILpopulation the DH has more advantages in terms of population characteristics and creation costs. |
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| Keywords: | Maize DH RIL BC HTP marker Inheritance law |
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