导入外源总DNA获得大豆早熟新品系

本文报道了在大豆自花授粉后，利用其形成的花粉管通道，将提取的含有早熟血缘供体大豆绥农８号的总ＤＮＡ，直接导入受体大豆黑农２６号中。在后代Ｄ２代中获得３个早熟株系，熟期比受体提早１５天，并迅速稳定。经异地鉴定，Ｄ９０－１０７２品系产量比对照品种提高１９．１％，于１９９３年进入省区试。经过对该组合的受体，供体及转化后代进行ＲＡＰＤ分析，证明供体ＤＮＡ片段进入受体引起后代基因组的变异。

Early Maturing Lines of Obtained through Foreign Total DNA Introduction in Soybean

This study reports that foreign DNA was introduced into recipient Heinong 26 directly through pollen tube after self-pollination of soybean. The donor was Suinong 8 containing early maturing consanguinity. Three early maturing plant lines were obtained in D2 generation and they matured 15 days earlier than that of the recipient. The lines stabilized fast. According to the experiment results in different locations the yeild of line D90-1072 was 19.9% higher than CK and was assigned into provincial local trial in 1993. Through analysis of RAPD (Random Amplified Polymorphic DNA) to the recipient, donor and offspring of the combination , it was proved that fragments of donor DNA have gone into the genome of the recipient and caused variation in the offsprings.